Effects On The Proliferation And Apoptosis Of Ovary Cancer Cell Line SKOV3Co-cultured With Lymphocytes Infected By Recombinant Adenovirus Ad-IL-12

Objective: To investigate the effects of lymphocytes infected byrecombinant adenovirus Ad-IL-12on the proliferation and apoptosis ofovary cancer cell SKOV3.Method: The peripheral blood lymphocytes were infected byrecombinant adenovirus Ad-IL-12and Ad-GFP for48h respectively.TheSKOV3cells were co-cultured with lymphocytes infected with Ad-IL-12(SKOV3/Ad-IL-12), lymphocytes infected with Ad-GFP (SKOV3/Ad-GFP)separately, and the untreated cells (SKOV3) were set as another control.IL-12double-subunits (P35, P40) mRNA expressions were tested byRT-PCR and the levels of IL-12P70protein in the cell culture supernatantwere detected with ELISA. The proliferation potential of SKOV3wasassessed by CCK8assay, flow cytometry was used to detect the cell cycleand cellular apoptosis, RT-PCR and Western blot were performed to detectthe expressions of BCL-2and Survivin.Results: The human recombinant adenovirus Ad-IL-12could successfully infect human peripheral blood lymphocytes and effectivelyprompt the expression and secretion of IL-12P70proteins; growth abilityof SKOV3cells (SKOV3/Ad-IL-12) was significantly inhibited (P<0.05).In addition, the percentages of SKOV3cells in the G1phase and theapoptosis (SKOV3/Ad-IL-12) were increased significantly (P<0.05), theexpression of BCL-2and Survivin were down-regulated（P<0.05）.Conclusions: The recombinant adenovirus Ad-IL-12could effectivelyinfect the human peripheral blood lymphocytes and express the IL-12P70protein successfully, which cocultured with SKOV3cells could inhibit theproliferation of SKOV3cells by blocking cell cycle and inducingapoptosis.