| Ovarian cancer is one of the most common gynecologic malignant tumors,the mortality rate of which ranks first in gynecological tumors.Although there have been many studies on ovarian cancer at home and abroad,the etiology and pathogenesis have not yet been clearly understood.Due to imperceptible early symptoms and lack of effective treatment in advanced medical records,ascites and distant metastasis are usually found in the majority of patients diagnosed with ovarian cancer.Although living quality of ovarian cancer patients with advanced ovarian cancer can improve significantly through surgery and chemotherapy,but in 2017,the USA National Institute survey:the five-year survival rate of ovarian cancer is 45.6%[1].The long-term survival rate is lower.At present,ovarian cancer has become a serious threat to the majority of women's life and health of the most important gynecological tumors.2012,scientists of Nature have found that:some ovarian epithelial carcinoma originates from two kinds of epithelial tissue of the teansition zone,the other from epithelial tissue stem cells[2].Therefore,it is still the hotspot and difficulty of ovarian cancer research on relevant mechanisms of the occurrence and development of epithelial ovarian cancer,so as to find molecular markers and effective therapeutic targets[3-4].IP6(Inositol hexaphosphate)is a natural compound composed of inositol and 6 phosphate ions,molecular structure including muscle and 6 phosphate ions.IP6 is rich found in the form of phytin in yeast,actinomyces,mammalian and plants[5],it's anti-cancer effect has been demonstrate in the experiment in vivo and in vitro.but the mechanism of such effect has not been fully understood.and has become a hotspot of anticancer drugs in recent years.In vivo and in vitro researches on malignant tumor of colon cancer,lung cancer,breast cancer,etc have proven that IP6 has efficient anticancer effect and has synergistic effect with inositol,thus showing a more potent anticancer effect.At present,the main mechanism of IP6 involved antitumor specific cytotoxicity effect,promoting apoptosis of cancer cells,cytotoxic effect,reinforcing the DNA reparative ability,telomerase inhibition and antioxidant effect[6-8] and so on.RB gene is a tumor suppressor gene,which exists in the nucleus in dephosphorylation of activated form and inactivation of phosphorylated form.Activated Rb protein will block the entrance of cells from G0/G1 to S phase.So the inactivation of Rb gene will keep cell in a period of continuous proliferation,which may cause malignant transformation.Aberrant cell cycle regulation has been confirmed to be an important mechanism in the tumor development.As one of the important regulatory factor in the critical G1/S checkpoint of the cell cycle,the abnormal expression of cyclin D1 may induce cancer cells into the cycle of multiplication and eventually leading to the occurrence of thetumor.Objective: The experiment object is human ovarian serous papillary cystadenocarcinoma SKOV3 cells,giving IP6 different measurement used alone and IP6 with cisplatin together,to investigate the inhibitory effect of inositol IP6 on human ovarian serous papillary cystadenocarcinoma SKOV3 cells and observe the changes in morphology and quantity of SKOV3 cells as well as the expression of Rb and CyclineD1 protein,so as to provide the reference for the diagnosis and trentment of ovarian cancer and clinical practice of IP6 on epithelial ovarian cancer.Methods:1 Cell culture : Human ovarian carcinoma SKOV3 cells are cultured in RPMI-1640 medium(containing 10% fetal bovine serum).Put culture bottles in temperature of 37,containing 5%CO2.?2 Preparation of experimental drugs: Experimental drugs were prepared to the required concentration with 0.9% sodium chloride injection.3 The concentration of the drug used in the experiment and the preparation of the drug:Blank control group: fresh medium,IP6 + Ins low dose group: 1.5mmol/L,middle dose group 3mmol/L,high dose group 6mmol/L,cisplatin concentration 3ug/ml,combined group :concentration cisplatin: 3ug/mL+(IP6+Ins): 3mmol/L.4 Experiment group:culture medium were randomly divided into 6 groups:blank,IP6 low medium and high dose group,cisplatin group,joint group(IP6+cisplatin)5 Rb gene expression in tumor tissues was detected by Real Time-PCR.6 Western Blot assay was adopted to detect the expression of CyclinD1 in tumor tissues.7 Statistical methods: SPSS13.0 statistical software was used to process the experimental data.P<0.05 was considered statistically significant.Results:1 Expression of Rb in ovarian cancer cells: Rb expression was detected out in all groups.The expression was low in the control group while was high in the experimental groups.The order of the expression was: the combination group>the cisplatin group>high dose group>medium dose group>low dose group>the control group.The difference was statistically significant(P<0.05).2 Expression of CyclinD1 in ovarian cancer cells: Cyclin D1 expression was detected out in all groups.The expression was high in the control group while was low in the experimental groups.The order of the expression was: the combination group<the cisplatin group< high dose group<medium dose group <low dose group<the control group.The difference was statistically significant(P<0.05).Conclusion:1 Both IP6+inositol and cisplatin showed inhibition effect on the tumor,which were all dose dependent and the two act in synergistic manner.2 IP6+inositol could increase the expression of Rb and decrease the expression of CyclinD1,which showed a dose-dependent characteristic. |
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