Timing Chronic Restraint Stress Induced The PMDD Liver Depression Syndrome Rat Model Validity Estimated And Preliminary Study On The Intervention Mechanism Of Shu Yu Capsules

Objective: Explore of PMDD liver depression syndrome model in ratsstandardized methods, to further improve the quality assessment of the ratmodel; Using the animal models as the carrier, and setting a blank controland a positive control to study the shu Yu capsule intervention effect.Detection in peripheral blood of animals and central brain regions keyindicators content to explore the possible mechanism of action of Yu ShuCapsule.Methods: Used the timing and continuous chronic restraint stress to preparePMDD liver depression syndrome rat model and applied fluoxetine drugintervention. Acquisition model animal behavior and biochemical indexes ofthe two methods. Contrasted the different effects of the two methods andestimated the validity of the model animal (including face validity, constructvalidity, predictive validity), to standardize the method of PMDD liverdepression syndrome rats model and preliminary assessment on the quality ofthe model.Applied the timing chronic restraint stress to prepare PMDD liverdepression syndrome rat model. Acquisition of rats in each group in peripheralblood and the frontal lobe, hippocampus, hypothalamus three brain regions,HPLC was used to detect different brain regions of the rat central monoamineneurotransmitters (NE,5-HT,DA), Measured by radioimmunoassay in serum sex hormone-regulating hormone content(P,PRL,E2).Explore Shu Yu Capsulemechanism of intervention of PMDD liver depression syndrome rat model.Results:(1) By rats after modeling and biochemical detection, the timingchronic restraint stress model rats’ key behavioral indicators: locomotoractivity in the open-field test, sucrose consumption in the sucrose preferencetest and OT%and OE%in the Elevated Plus-Maze test decreased significantlycompared with the normal group in the non-acceptance period. Correspondingbiochemical indicators: Body weight gain, Adrenal Gland,5-HT content in serum,were significantly different with the normal group. While continuous chronicrestraint stress model rats, just individual indicators: locomotor activityin the open-field test, Body weight gain, Adrenal Gland,5-HT content in serum,were significantly different with the normal group. The key indicators:sucrose consumption in the sucrose preference test and OT%and OE%in theElevated Plus-Maze test, there were no significant differences compared withthe normal group. As estimating by the model validity, we know the timingchronic restraint stress modeling method induced PMDD liver depressionsyndrome rats, regardless of face validity, construct validity, or predictivevalidity can better simulate clinical manifestations of the disease.(2) The timing chronic restraint stress model rats’biochemicalindicators show that: Compared with the normal group, the model group rats’NEcontent in the hypothalamus, hippocampus, frontal lobe significantlydecreased (P<0.01,P<0.01,P<0.05), DA content in the hippocampus,hypothalamus, and5-HT content in the hippocampus, hypothalamus, frontal lobealso significantly reduced (P<0.01, P<0.01)(P<0.01, P<0.01, P<0.01); At thesame time, the P content and E2content in the serum was significantly lower(P<0.01, P<0.05). After the Shu Yu capsules intervention, the indicators wereincreased significantly restored to normal levels.Conclusion:(1) The timing chronic restraint stress method is better thanthe continuous chronic restraint stress method,using the former method induced rat model of PMDD liver depression syndrome has good face validity,construct validity and predictive validity, suggesting that the preparedmodel animal behavior and biochemical indicators change clinical prototypemore consistent.(2) After the Shu Yu capsules intervention, compared with the modelgroup,the treatment group behavior and biochemical indicators have improved.The NE content in the Hypothalamus, hippocampus, frontal lobe, DA content inthe hypothalamus, hippocampus,5-HT content in the hypothalamus, hippocampus,frontal lobe and the P, E2content in the serum increased significantly andclose to the normal level. Suggesting the Shu Yu capsule function key brainregions may be the hypothalamus, hippocampus and frontal lobe, particularlythe hypothalamus, hippocampus are more closely related; The mechanism ofaction may be related to regulating body of monoamine neurotransmitters, sexhormones and regulation of hormones.