The Expression Of PHB, SNRPB In Squamous Cell Carcinoma/Adenosquamous Carcinoma (SC/ASC)of Gallbladder And Adenocarcinoma(AC) Of Gallbladder

Objective:To study the expression levels of PHB, SNRPB in squamous cell carcinoma/adenosquamous carcinoma (SC/ASC) and adenocarcinoma (AC) of gallbladder and its clinical pathological significance of organization, comparing the clinical pathological features of SC/ASC and AC.Methods:There are46cases of SC/ASC and80cases of AC. PHB and SNRPB expressions were detected with EnVision immunohistochemistry.Results:There are more patients that<45years old and with poorly differentiated adenocarcinoma in AC than SC/ASC (P<0.05), while there are more patients with maximum diameter of the tumor>3cm in SC/ASC than AC (P<0.05). Gender, presence of gallbladder stones, TNM staging, lymph node metastasis, invasion of surrounding tissues or organs, surgical procedure, the average survival time and the positive expression of PHB and SNRPB showed no significantly difference between AC and SC/ASC (P>0.05). Patients with tumor maximum diameter<3cm, TNM staging â… +â…¡ stage, no lymph node metastasis, no invasion of surrounding tissues and organs and accepting radical excision had significantly lower PHB expression than that with tumor maximum diameter>3cm, TNM staging IV stage, lymph node metastasis, invasion of surrounding tissues and organs, and non-radical resection in SC/ASC (P<0.05); the expression of SNRPB was contrary (P<0.05or P<0.01). The expression of PHB and SNRPB has no obvious relation with other clinical pathological characteristics in SC/ASC (P>0.05). Patients with high differentiation, tumor maximum diameter <3cm, TNM â… +â…¡ stage, no lymph node metastasis,, no invasion of surrounding tissues and organs accepting radical excision had lower PHB expression than that with low differentiation, tumor maximum diameter>3cm, TNM â…£ stage, lymph node metastasis, invasion of surrounding tissues and organs, and non-radical resection in AC (P<0.05or P<0.01); the expression of SNRPB was contrary (P<0.05or P<0.01);Patients without gallbladder stone had higher SNRPB expression than that with gallbladder stone (P<0.05); PHB and SNRPB expression has no obvious relation with other clinical pathological characteristics in AC (P>0.05). Kaplan and Meier survival analysis showed that the degree of differentiation, tumor maximum diameter, TNM staging, lymph node metastasis, invasion of surrounding tissues and organs, operation method and PHB and SNRPB expression had close relationship with survival time of SC/ASC and AC(P<0.05or P<0.01); the survival time was significantly less in patients with positive PHB expression than that with negative expression (P SC/ASC=0.006, PAC=0.000), but the positive expression of SNRPB was contrary (PSC/ASC=0.000, PAC=0.000); Cox multi-factor analysis showed that poorly differentiation, tumor maximum diameter>3cm, TNM staging â…¢ or â…£ stage, lymph node metastasis, invasion of surrounding tissues and organs, non-radical resection and PHB positive or SNRPB negative expression and negatively correlated with postoperative survival rate, positively correlated with mortality, Above-mentioned risk factors are independent prognostic factors in SC/ASC and AC.Conclusion:Compared with AC, the clinical pathological features of SC/ASC have no obvious difference except the onset age, degree of differentiation and tumor size; the expression of PHB and SNRPB reflects the occurrence, progression, and clinical biological behavior and prognosis in SC/ASC and AC (from the opposite side). The patients with positive expression of PHB or (and) the negative expression of SNRPB have poor prognosis in SC/ASC or AC, both are important biological markers.