| BackgroundPost-stroke depression (PSD) is one of the most common complications after stroke. PSD is a series of mood or affective disorder and its one of the main symptoms is depressi-on. The status of depression directly affects the patient’s quality of life, causes the pati-ent’s cognitive impairment, these factors are not conducive to the primary disease rehabilit-ation. Both NPAS4(neuronal PAS domain protein4) and ARNT2(aryl hydrocarbon receptor nuclear translocator2) are the member of basic helix-loop-helix,(bHLH)-PAS (PER-ARNT-SIM) transcriptional regulators superfamily. These expressions may further impact on cognitive function. Both expressions might influence cognitive function of PSD patients by regulating and controlling the function of brain-derived neurotrophic factor (BDNF).Objectives1To study the Relationship between the expressions of ARNT2and NPAS4and cognitive impairment in Post-stroke depression patients2To analyze the differences of ARNT2and NPAS4between Post-stroke depression patients and patients with primary depression.Methods1. Stroke was diagnosed according to2010china diagnosis and treatment guidance for Acute ischemic stroke, depression diagnostic criteria met the Diagnostic and Statistical Manual of the American mental disorders4th edition (DSM-Ⅳ), depression was evaluated by using24-item Hamilton Depression scale (Hamilton).I divided them into four groups, they were PSD group, the stroke group, the primary depression group and the healthy control group.2. Evaluated cognitive function by event-related potential (ERP) P300.3. Detected the expression of NPAS4, ARNT2mRNA from peripheral blood mononuc-lear cells by using real-time quantitative PCR in post-stroke depression group, the mere str-oke group, the primary depression group and healthy control group.Results1. Event-related potential (ERP) P300.1) The latency of P300:Compared with the control group, the latency of P300in PSD group, the stroke group and the depression group were longger and the differences were statistically significant (P<0.01) between untreatment and after two weeks of treatment, compared with the stroke group and the depression group, the PSD group were longger and had significant statistically difference (P<0.01). After two weeks of treatment, the P300latency in PSD group, the stroke group and the depression group were shorter than before treatement, and had statistically significant difference (P<0.01).2) The amplitude of P300:Compared with the control group, the amplitude of P300in the PSD group, the stroke group and the depression group were lower, and had statistically significant differences (P<0.01) before treatment and after two weeks of treatment, compared with the stroke group, the PSD group were lower, and had significant statistically difference (P<0.01), There is no statistically significant difference between the PSD group and the depression group (P>0.05). After two weeks of treatment, the amplitude of P300in PSD group, the stroke group, depression group were significantly higher than untreatment, and had statistically significant differences (P<0.01).2. The levels of ARNT2and NPAS4was detected by real-time PCR1) The mRNA expression level of ARNT2gene in post-stroke depression, stroke group,the primary depression group and healthy control group, CT values>35. This is suggest the level of ARNT2gene in human peripheral blood was very low, almost no expression.2) The mRNA expression level of NPAS4gene:Compared with the control group. Before treatment the relative mRNA expression level of NPAS4in PSD group and depression group were descended,the difference was statistically significant (P<0.01), There is no statistically significant difference between the PSD group and the depression group (P>0.05).the relative expression level of NPAS4mRNA in stroke group was significantly higher than the PSD group, depression group and normal control group, the difference was statistically significant (P<0.01).After two weeks of treatment, the relative expression level of NPAS4mRNA in PSD group, stroke group and depression group were significantly higher than in the normal group,and had significantly statistical differences (P<0.01), The level of NPAS4gene in PSD group was higher than the depression group, the difference was statistically significant (P<0.01), there was no statistically significant difference between PSD group and the stroke group (P>0.05).Before treatment and after two weeks of treatment in each group to compare:In PSD group,the stroke group and depression group, the differences were all statistically significant (P<0.01).3. Pearson Correlation Analysis:Between P300latency and amplitude presented a negative correlation (r=-0.438, P=0.000), Between NPAS4and P300latency also presented a negative correlation (r=-0.394, P=0.000).Conclusions1. Between NPAS4and P300latency presented a negative correlation, and NPAS4correlated with cognitive function. The transcription factor NPAS4participated in the regulation of cognitive function.2. The relative mRNA expression level of NPAS4in PSD group and depression group were difference. |
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