| Purposes:By observing the weight,behavior and the change of immune cell factors of the rats with chronic fatigue under the treatment of scalp-acupuncture, the paper explores the effect and mechanism of action of scalp-acupuncture on chronic fatigue syndrome, thus providing ideas and basis for clinical treatment of chronic fatigue syndrome.Methods:Seventy SPF healthy SD male rats were randomly divided into normal group, model group, restraint group and acupuncture group. The model of rats with chronic fatigue syndrome was established by weight-bearing swimming exhaustion and chronic stress. The rats of acupuncture group were treated by scalp-acupuncture at Baihui acupoint, emotion area, and bilateral sensory areas for30minutes each time, once per day, for15days; the rats in the restraint group were bundled for the same period of time with that of the acupuncture group; the rats in the normal group and model group were not dealt with. The general condition, behavior and the change of indicators IL-1β、IL-6and TGF-β1of immune cytokine of rats were observed.Results:1. The general state shows:before the experiment, the difference of weight of the rats between different groups is not statistically signif icant(P>0.05). Before treatment(after model building), the weight of rats in model group, restraint group and acupuncture group is significantly lower, compared with the weight of rats in normal group, and he difference is statistically signif icant(P<0.01). After treatment, the weight of rats in model group and restraint group is apparently lower than that of rats in normal group, and the difference is statistically significant(P<0.01).The difference of weight of rats between model group and restraint group is not statistically significant (P>0.05).Ethological detection:①morris water maze experiment:before treatment (after model building), escape latency of the rats in the model group, restraint group and acupuncture group is prolonged significantly and times of crossing platform are decreased significantly, compared with that of the rats in normal group. The difference is statistically significant. After treatment, the escape latency of rats in the model group and restraint group is obviously longer, and the times of crossing platform are obviously decreased, compared with that of the rats in normal group. The difference is statistically significant(P<0.01). The escape latency of rats in the acupuncture group is obviously shortened and the times of crossing the platform are increased, compared with that of the rats in the model group. The difference is statistically significant(P<0.01). The difference between the model group and the restraint group is not statistically significant (P>0.05).②the open field experiment: before treatment (after model building), the horizontal and vertical activities of the rats in the model group, restraint group and acupuncture group are significantly decreased, compared with that of the rats in the normal group. The difference is statistically significant (P<0.01). After treatment, the horizontal and vertical activities of the rats in the model group and the restraint group are significantly decreased, compared with that of the rats in the normal group. The difference is statistically significant (P<0.01). The horizontal and vertical activities of the rats in acupuncture group are obviously increased, compared with that of the rats in the model group. The difference is statistically significant (P<0.01). The difference between the model group and the restraint group is not statistically significant (P>0.05)3. The result of immune cell factors shows:IL-1β,IL-6and TGF-β1content in the serum of rats in the model group and restraint group increases significantly, compared with that of the rats in the normal group. The difference is statistically significant(P<0.01). IL-1β,IL-6and TGF-β1content in the serum of rats in the acupuncture group decreases obviously, compared with that of the rats in the model group. The difference is statistically significant (P<0.01). The difference of IL-1β,IL-6and TGF-β1content in the serum of rats between the model group and restraint group is not statistically significant(P>0.05).Conclusion:1. The CFS rat model can be successfully established by a combination of weight exhaustion swimming and unpredictable chronic stress, and the rat model has certain stability.2. Scalp-acupuncture can improve the state of physical and psychological fatigue of the model rats by increasing weight, enhancing the ability of learning and memory, increasing independent activities, and relieving depression and anxiety3. Scalp-acupuncture can adjust the immune function of the CFS model rats. Regulating immune function may be one of the mechanism of treating chronic fatigue syndrome by scalp-acupuncture. |
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