Yinxieping Pill Is Impacted That Imiquimod-Induced Psoriasis-Like Skin Inflammation In Mice Serum IL-17and IL-23Expression

Objective:Construct the BALB/c mice back model of psoriasis and detect the change of morphology, histopathology, IL-17and IL-23in BALB/c mice back to explore the possible mechanisms of Yinxieping pill to treat psoriasis.Methods:52BALB/c mice were randomly divided into six groups, each group of eight. The Blank controll group, model control group, Chinese medicine high, medium and low dose group, tripterygium wilfordii glycosides.Another4mice only preliminary experiments,Then the evaluation model method was successful.Outside the blank control group with vaseline, and the rest group with imiquimod5%cream to mouse back skin for8days,one time a day.Using this method to establish model mice psoriasis samples, and evaluate building model control group mice. While each group of mice treated with the drug orally, blank control group, model control group received saline, the rest of the group were to be Hook, Yinxieping pilll high, medium and low-dose orally for8days, and one time a day. After the last administration, observed BALB/c mouse dorsal skin PASI score; blood through the eyeball method to detect cytokines in serum; take back pathological tissue sections were relatively organizational learning. Throughout the experiment, generally mice closely observed. Results:1There were no significant differences in each group and Yinxieping pill Tripterygium glycosides group of psoriasis-like BALB/c mouse back skin PASI score (P>0.05), and compared with the model group and control group scores were significantly different (P<0.05).2Groups psoriasis-like BALB/c mouse peripheral IL-17and IL-23were significantly higher than the control group (P>0.05). The Yinxieping pilll high, medium and low-dose group and Tripterygium glycosides group were significantly different with the model group content in peripheral blood (P<0.05).3For psoriasis-like BALB/c mouse back skin pathological changes,psoriasis flat pill group and Tripterygium glycosides groups are significantly different and the model group and control group (P>0.05).Conclusion:1.A serum psoriasis-like mouse model of IL-17and IL-23expression levels higher than the control group, indicating that high expression of IL-17and IL-23in a mouse model of psoriasis-like description of immune abnormalities, confirmed in the pathogenesis of psoriasis and IL-23, IL-17related.2.The Yinxieping Pill has a lower level of psoriasis psoriasis-like role model mouse serum IL-17, IL-23levels. 3.The Yinxieping Pill mouse model mice by inhibiting the mechanism of action may be IL-17, IL-23, suppressing immune and inflammatory response, play alleviate psoriasis-like mouse model of psoriasis-like lesions occurred the role of development.