Studies On The Extraction Process And Quality Control For Chelidonium Majus L

Object:The chemical composition of Chelidonium majus L. was made by alkaloids and non-alkaloids, which contains a variety of isoquinoline alkaloids is the main active ingredient. Used in the clinical treatment of stomach, cough, whooping. So the topic optimized the extraction process for the total alkaloids of Chelidonium majus L. and established its quality control method.Methods:In this study, Chelidonium majus L. were extracted by acidic alcohol reflux, to include total alkaloids content and yield of total alkaloids as the evaluation indicators, Box-Behnken design was adopted to arrange test, response surface methodology was used to analyze the results of the test. In order to control the quality of the alkaloids, the content of the alkaloids was determinated by Ultraviolet Spectrophotometry, Chelidonine, Chelerythrine and Berberine were identified by thin layer Chromatography and determinated by high performance liquid Chromatography.Results:The optimum extraction process was90%acid(0.25%hydrochloric acid, V/V)ethanol extraction, extracted three times, each time50min, solid-liquid ratio of l0ml/g. The extract liquid was concentrated, cleaning, extracted and dried into the extract. The paper developed the identification methods of Chelidonine, Chelerythrine and Berberine. The determination methods of Chelidonine, Chelerythrine and Berberine in the alkaloids. Methodological study test were all qualitified.Connlusion:The extraction process for Chelidonium majus L. was rational, stabile and reliable, the quality control for Chelidonium majus L. was accurate, fast, simple, reproducible, and good separation, could be used for control the quality of the alkaloids of Chelidonium majus L.