Study On Quality Evaluation Of Demesticated Chelidonium Majus L.

In this paper,the extraction methods of alkaloids and the dynamic accumulation of chemical constituents were studied,also the effects of different drying methods and different soil types on the quality of Chelidonium majus L.were discussed,which in order to provide reference for the timely harvesting,high quality and efficiency production and domesticated cultivation management.Based on the results of transcriptome sequencing,three key enzyme genes were identified in the alkaloid synthesis pathway,and the expression levels of the three key enzymes were analyzed at different stages,which laid the foundation for the study of differentially expressed genes.Based on the results of transcriptome sequencing,three key enzyme genes were identified in the alkaloid synthesis pathway,and the expression levels of the three key enzymes were analyzed at different stages,which laid the foundation for the study of differentially expressed genes.The main contents and results are as follows:1.The active ingredients of Chelidonium majus L.are mostly alkaloid compounds,in view of the extracted efficiency of the alkaloid compounds,three methods and extracted solvent were compared,and the orthogonal experiment was used to determine the optimum extraction process.The results showed that 0.5% hydrochloric acid added to the methanol solution was the best solvent,the ratio of material to liquid was 1:50,and the extraction time was 1h,with which condition the extraction efficiency was the best.2.The result of study on the accumulation dynamics of effective components in the growth and development of Chelidonium majus L.are as follows: The effects of various active alkaloids including protopine,chelidonine,sanguinarine,chelerythrine,berberine,coptisine in whole growth and development of Chelidonium majus L.were observed.The results showed that the dynamic changes of the components in different growth stages were different.To determine the appropriate harvest period of Chelidonium majus L.: Selecting the indicators is closely related to the quality of Chelidonium majus L.,the harvest time of the ground part of of Chelidonium majus L.is in July and the underground part of Chelidonium majus L.is in October.3.Effects of different drying methods on quality formation of Chelidonium majus L.:The effects of drying in the sun,drying in the shade and drying at different temperatures on the quality of Chelidonium majus L.The results showed that different drying methods can make the appearance and moisture of Chelidonium majus L.meet 2015 version of "Chinese Pharmacopoeia" provisions,but there are differences in herbal quality.Considering the content of active ingredients with comprehensive analysis,60 ℃ drying can replace drying in the sun or in the shade which were recorded in pharmacopoeia.4.Effects of different soil types on the quality of Chelidonium majus L.:The differences in the contents of alkaloids of Chelidonium majus L.which were planted in the loess,muck and Ginseng soil were compared,and the difference was not significant.Therefore,it is suggested that Chelidonium majus L.can be used as one of the medicinal plants which can be planted after planting ginseng.5.Based on the transcriptome data,three genes tyrosine decarboxylase,polyphenol oxidase,primary amine oxidase and primary amine oxidase were selected from the biosynthetic pathway of the alkaloid in Chelidonium majus L.Through the study of the amplification curve and the solubility curve,the method of fluorescence quantitative PCR was established,the expression levels of tyrosine decarboxylase,polyphenol oxidase and primary amine oxidase in different periods were obtained,which laid a foundation for the study of the biosynthesis mechanism of alkaloid constituents in Chelidonium majus L.