Method For The Determination Of Acanthopanax Senticosus Extract Fingerprint And Multilingerdient Content

Acanthopanax is the dry root、rhizome and stem of Harms o f Radix Acanthopanax senticosus (Rupr.et Maxim) Harms.It has a little bitter and acrid flavor,mild attribute,non-poisonous.For a sdthenic splenonephro-yang, physically weak fatigue, inappetence, aching pain of waist and knees and insomnia and dreamful slee p ect, as one of the commonly used clinical traditional Chinese medicine. Acanthopanax mainly distributed in China’s northeast, north, far east area of Russia, Hokkaido of Japan and North Ko rea, etc. Wild resources of our country is more substantial.In this paper, the Acanthopanax Extrac quality standards we re detailed, comprehensive study, on the foundation of original standard, establishment of Acanthopanax Extrac fingerprinting m ethods, fingerprint identification using the method of HPLC,30%acetonitrile-0.2%phosphoric acid as the mobile phase, grad ient elution.In60minutes,9degrees of separation good chroma tographic peaks.Through the similarity calculation of chromatogr aphic fingerprint of traditional Chinese medicine Acanthopanax Extrac sample similarity, consistent with the fingerprinting requi rement.The Acanthopanax Extrac fingerprinting methods on the basi s of the27batch of Acanthopanax Extrac samples were Syringi n, Eleutheroside E, isofraxidin, chlorogenic acid and protocatech uic acid content determination. and through methodology examin ation respectivly, proving the methods are both convenient and f easible,having favourable reproducibility and accuratility, To ma ke more comprehensive content determination of Acanthopanax e xtract.The main purpose of this study is to further improve the q uality standard of Radix Platycodon grandiflorum, to better cont rol their intrinsic quality.