Early Anti-platelet Aggregation Effect And Mechanism Of Endothelial Nitric Oxide Synthase Gene-modified CD34~+Cells

Objective:To evaluate early stage anti-platelet aggregation effect of nitric oxide synthase genemodified-CD34+bone marrow stem cells in vitro,and to explore its mechanism.Methods:Extracted bone marrow from canine,isolated CD34＋cells by immunomagnetic,transfected endothelial nitric oxide synthase (eNOS) gene to CD34＋cells withliposomes,endothelial cell growth factor induced and amplified.Detected theexpression of tissue factor (TF) stimulated by lipopolysaccharide (LPS),and performplatelet adhesion assay to detect the anti-platelet aggregation effect of eNOS-CD34+cells in vitro.Results:Platelet adhesion assay in vitro showed that the inhibition of platelet aggregation ineNOS-CD34+cells was significantly higher than that in non-transfected group. AfterLPS stimulation, TF immunofluorescence of eNOS-CD34+cells was significantlyweaker than that in non-transfected group.Besides, Western blot detected that theexpression of TF protein in eNOS-CD34+cells was significantly weaker than that innon-transfected group.Conclusion:eNOS gene transfection significantly increases anti-platelet aggregation effect ofCD34+bone marrow stem cells in vitro, its mechanism may be related to the inhibitionof TF expression.