Expression Of Cx43and GATA-4in PCOS And Intervention Effect Of Compound Ganshao Patch On Them

Objective: Polycystic ovary syndrome (PCOS) is a common female ovulation disorders, clinicalmanifestations of oligomenorrhea, infertility, hirsutism, obesity, etc.. so far the cause unknown, theclinical therapeutic effect of poor. Cx43is connected to the cell membrane surface protein particles.GATA-4is a transcription factor. June animal studies found that Cx43and granular cells in normaldevelopment of. White found the proliferation and differentiation of GATA-4cells is closely relatedwith particles. Two the normal development and follicle associated. Study on Cx43and GATA-4expression in ovary and compound PCOS model rats Ganshao patch intervention mechanism. Theformer research results, compound Ganshao patches in the treatment of PCOS model rats, the serumlevels of sex hormones, light microscope and electron microscope histological changes are similar tothe clinical PCOS recommended drugs with the effect of Diane-35.Expression of Cx43andGATA-4in PCOS and intervention effect of Compound Ganshao Patch on them.Methods: Polycystic ovary rat models were established by letrozole.Forty-eight SDfemale rats, aged6weeks, were used and randomly divided into two groups:normal group andexperiment group. The blood was collected from vena cava, centrifugal settling,the serum wastaken. sex hormone was tested by chemoluminescence method. Experiment group were used andrandomly divided into three groups: model group, Diane-35group and Compound Ganshaopatch group.The blood was collected from vena cava, centrifugal settling,the serum was taken.sex hormone was tested by chemoluminescence method.One of the model group and the35group has death due to improper operation.there are46. Double ovaries of all thegroupwereresected and were put into10%formalin for24hours. And then, The expression of Cx43andGATA-4by immunohistochemical and by Westernblotting in ovary tissue ingroup withpolycystic ovaries induced by Compound Ganshao. Using SPSS17.0statistical software foranalysis; data are presented as mean±standard deviation said, diversity Honma compared withsingle factor analysis of variance, two two compared with LSD-t test between two groups werecorrelation analysis using Pearson correlation. The difference significance statistics while P <0.05.Result: Polycystic ovary rat models were established by letrozole.Light microscopically:the normal control group tissue into polycystic change, observation modelgroup serum LH, FSH,T, the expression of（2.41±0.23）IU/L，LH（8.11±0.85）IU/L，T（2.3±0.96）nmol/L，by model groupgroup were higher than the FSH（2.95±0.21）IU/L，LH（8.72±0.99）IU/L，T（3.18±1.46）nmol/Lby normal control group,(P＜0.05). (1)The average expression of Cx43in PCOSgroup and normal control group was (98.19±6.51) and (149.59±9.25), respectively, and there was a significant difference between themby immunohistochemical (P <0.05).The average expression of Cx43in PCOS rat s andCompound Ganshao patch group was (98.19±6.51) and (144.28±4.72), respectively, andthere was no significant difference by immunohistochemical (P>0.05).(P <0.05).The averageexpression of Cx43in Diane-35group and Compound Ganshao patch group was (143.79±9.81)and (144.28±4.72), respectively, no significant difference by immunohistochemical (P>0.05).(2)The average expression of GATA-4in normal control group and PCOS rat s was (129.37±9.99) and (162.91±9.38), respectively, and there was a significant difference between them by immunohistochemical (P <0.05).The average expression of GATA-4in PCOS rat sand Compound Ganshao patch group was (162.91±9.38) and (115.13±7.04), respectively, andt here was a significant difference between t hem by immunohistochemical (P <0.05).compaired with The expression of GATA-4(112.37±7.90)by Diane-35and CompoundGanshao patch group (115.13±7.04), the level of GATA-4was was higher,the others were notchanged by immunohistochemical (P＞0.05).(1)The average expression of Cx43in PCOS rat s and normal control group was(0.66±0.15) and (0.86±0.10), respectively, and there was a significant difference between them by Westernblotting.(P <0.05).The average expression of Cx43in PCOSgroup andCompound Ganshao patch group was (0.66±0.15) and (0.86±0.10), respectively, and therewas a significant difference between them by Westernblotting.(P <0.05).The averageexpression of Cx43in Diane-35group and Compound Ganshao patch group was (0.88±0.12)and (0.86±0.10), respectively, no significant difference by Westernblotting.(P>0.05).(2)The average expression of GATA-4in normal control group and PCOSgroup was(0.90±0.10) and （1.64±0.16）, respectively, and there was a significant difference between them by Westernblotting.(P <0.05).The average expression of GATA-4in PCOS rat s andCompound Ganshao patch group was（1.64±0.16) and （1.00±0.09）， respectively, and therewas a significant difference between them by Westernblotting.(P <0.05).compaired with Theexpression of GATA-4(1.15±0.32）by Diane-35and Compound Ganshao patch group （1.00±0.09）, the level of GATA-4was was lower,the others were not changed by Westernblotting.(P＞0.05).Conclusion: The development of PCOS may be closely related to the decreasing ofCx43and increasing of GATA-4in the ovary.the results indicate that the expression of Cx43inthe Compound of Ganshao patches was significantly higher than the PCOS rat. This studysuggests that the expression of GATA-4in the Compound of Ganshao patches was significantlylower than the PCOS rat.The suggesting that the levels of Cx43and GATA-4may be regulated via, improvement of follicular development, inhibition of apoptosis and play a therapeutic role.