Effect Of RPTS On Fas/Fasl Signal Pathway Of Apoptosis Induced In Human Gastric Cancer MKN-45Cell

ObjectiveIn this study, RPTS induced apoptosis of MKN-45cells would be tested.Meanwhile, the protein levels of caspase-3, caspase-8, Fas and FasL in theRPTS-treated MKN-45cells would be detected.MethodMKN-45cells were treated by RPTS of different concentrations for12h (0,5,10and20μg/mL).AO/EB and Hoechst33258fluorescent staining methods were used toobserve the cell apoptosis.The apoptosis rates of MKN-45cells treated by RPTS ofdifferent concentrations were analyzed by flow cytometry with Annexin V-FITC/PIstaining.The enzymatic activities of caspase-3and caspase-8were measured byspectrophotometry.The protein levels of Fas and FasL were detected by westernblotting.Results(1) Treatment with RPTS markedly induced the apoptosis of MKN-45cells in adose-dependent manner (0-20μg/mL).(2) The total apoptosis rate of MKN-45cells was26.06%, after treated with RPTS for12h.(3) Compared with the control group, the enzymatic activities of caspase-3andcaspase-8increased obviously after treated with RPTS for12h (P＜0.01).(4) The protein levels of Fas and FasL in the MKN-45cells were up-regulatedsignificantly with treatment by RPTS for12h (P＜0.01). Conclusion：(1) RPTS induced apoptosis of MKN-45cells.(2) RPTS increased the enzymatic activities of Caspase-3and Caspase-8.(3) RPTS up-regulated the protein levels of Fas and FasL.(4) RPTS induced apoptosis of MKN-45cells through targeting the Fas/FasL pathwayof death receptor pathway.