Studies On Biotransformation Of Two Active Sesquiterpenoids In Oleum Curcumae

Oleum curcumae, volatile oils extracted from the traditional Chinese medicine Rhizoma Curcumae with non-polar solvent, is potent in immunoloregulation, anti-cancer and anti-virus, etc., as the active constituent mainly consist of monoterpenoids and sesquiterpenoids, with curcumol and curdione as representative sesquiterpenoids.In this thesis, curcumol and curdione were isolated by silica gel column chromatography. Method was established to monitor the content of curcumol and curdione by High Performance Liquid Chromatography（HPLC）. Methodology studies showed satisfactory precision, stability, reproducibility and recovery. Assaying of curcumol and curdione by HPLC and TLC both indicted high purity of over 95％.Subsequently, Cunnighamella elegans AS 3.2028 capable of transforming curcumol was attained by screening over 20 strains of filamentous fungus. The transformed product of curcumol was prepared on a large scale and identified as the epoxidation product from the seven-memberd exocylic double bond of curcumol by IR, ¹³C-NMR, ¹H-NMR and EI-MS. Furthermore, it was found that Cunnighamella elegans AS 3.2028 could also cyclooxidize the carbon-carbon double bond of curdione, thus the transformed product was isolated and identified by the same spectral methods mentioned above.Finally, the optimum condition was identified for Cunnighamella elegans AS 3.2028 transforming curcumol and curdione respectively: for curcumol by resting cell with initial pH7.0 buffer（0.5mg/mL）at 28℃, and for curdione by growing cell with initial pH7.0 buffer（0.3mg/mL）at 28℃.