Correlation Study Of Notch3 Receptor And Jagged1 Ligand In Non-Small Cell Lung Cancer(NSCLC)

This paper focused on the expression and correlation of Notch3 and Jaggedl in lung carcinoma of clinical samples. Lung cancer is one of the most common cancers in the world. It is a leading cause of cancer death in men and women in the world. About 500,000 people died of lung cancer each year. Through the research of lung cancer, the translocation of chromosome 19 could be a cause of Notch3 overexpression,, which mainly cause lung carcinoma. In the development, Notch family plays a vital role in the Notch signaling pathway, which does not need a secondary messenger to fulfill the signaling pathway. But after maturation, the expression of Notch family decreased to the basal level. Many researches have showed that Notch family could correlate with many human diseases when they are not in their normal state. These diseases include: lung carcinoma, TALL (T-cell acute lymphocytotic leukemia), breast cancer, bladder cancer, urine carcinoma, ovarian cancer, CADASIL, Alzheimer disease, and etc. The causes of these diseases are complex. In some cases, mutation of Notch gene could be one reason, because with these mutations, the conformation of Notch could be changed, which will cause the signaling gateway changed from off to on. Or this conformation changing may provide hindrance, for example in CADASIL. Another reason is that the basal level of Notch is very low, while with this translocation or mutation, the expression of Notch increased and the signal is over amplified. Also another reason is that Notch signal may activate other signaling pathway, as c-myc, MAPK, and etc. In this paper, by immunohistochemistry(IHC) staining, lots of clinical data of 58 patient tissue samples showed that in lung adenocarcinoma and lung squamous cell carcinoma, the expression level of Notch3 and Jaggedl are quite high, which cause the over amplification of Notch signaling. Through the sequencing of Notch3 HD and PEST domain of 40 tissue and blood samples, there is no mutation in these two regions. So the cause of this disease is not mutation, but overexpression. Another experiment is Notch3 Sandwich ELISA, though we do not find Notch3 protein in patient serum, the result is exciting in that Notch3 could be a target for drug delivery.