The Antipyretic Effect Evaluation Of Water Baffulo Horn And Development Of Pearl Product

PartⅠ: The basic study and effect assess of water baffulo horn antipyreticactive compound.Cornu Bubali has the effect on reducing the temperature, detoxicating, coolingthe blood, arresting convulsion and so on. Contemporary study show that CornuBubali has conspicuous cardiotonic effect, has the effect on conscious-sedation,anti-convulsion, anti-inflammatory, anti-infection, protecting the liver and reducingthe blood-fat and so on. Cornu Bubali is mainly used to treat fever, inflammation,bleeding, convulsion, purpura, psoriasis clinically. This part, we mainly study theantipyretic active compound of water baffulo horn, and assess the effect of sedation,blood clotting and antipyretic.1．Comparison of the extraction methodCompare the method of reflux extraction, microwave extraction andhyperacoustic extraction through the protein contents and the yield. As the result,reflux extraction is the best way, and establish the best technology. Investigate therelationship between the number of times of extraction and the protein dissolution, weget the conclusion that the number of times should be more than 6．2．Pharmacodynamics research of water buffalo horn(1) Spontaneous movement experiment is used to investigate the sedation of waterbaffulo horn, it shows the sedation is unconspicuous.(2) Blood clotting experiment is used to investigat the thrombosis of water baffulohorn, the water baffulo horn can decrease the blood clotting time.(3) Pyretolysis experiment is used to investigat the antipyretic effect of water baffulohorn, the water baffulo horn can reduce the temperature of febrile rat. Pyretolysisexperiment is also used to investigat the antipyretic effect of ethanol extraction andwater extraction after the ethanol extraction, as a result, The antipyretic effect ofethanol extraction is unconspicuous, while the water extraction after the ethanolextraction is conspicuous, it suggests that the water-extraction is the main activecomponent. The pyretolysis experiment is used to select the optimal dose of baffulohorn water extraction, and the optimal dose is 4 times of the daily dosage.3．Selection on the antipyretic effect component of water buffalo horn waterextraction(1) Column chromatography is used to isolate the water baffulo horn water extraction into three parts: the part of high molecular, the part of mid molecular and the part oflow molecular.(2) Pyretolysis experiment is used to investigate the antipyretic effect of the isolations.As a result, the part of high molecular and low molecular have the antipyretic effect,and antipyretic effect of the part of mid molecular is unconspicuous.(3) Triscine-SDS-PAGE is used to calculate the relative molecular weight of the partof high molecular and mid molecular.As summed up, we can conclude: the main active component is water-solublesubstances, and has the effect on reducing the temperature and cooling the blood,the antipyretic active compound is polypeptide.PartⅡ: The investigation and development of new product compound pearlusing for Yin-deficiency postmenopausal osteopornsis1．The pearlPearl has a long history in Chinese medicine, and has certainly curative effect.There are two kinds of pearl, seawater pearl and freshwater pearl. Pearl has effects ofrelieving convulsion, calming the nerves, nourishing Yin, improving eyesight,detoxigying and promoting granulation. Pearl is mainly used to treat inflammation,exelcosis, ophthalmology disease clinically. Pearl contains calcium carbonates,keratins, amino acids, microelements, prophyrins, vitamin B etc. Pearl has the effecton anti-defatigation, anti-aging etc.2．Climacteric syndromeModern medicine consider that the reason of climacteric syndrome is low estrinlevel leading to the hypothalamus-hypophysis-ovary axis functional disorder.Clinically, there are hormone replacement therapy, chemotherapy, traditionalChinese medicine typing therapy and so on treating the climacteric syndrome.3．The development of new product for PMOPLarge Yin-Nourishing Pill contains pearl, can reinforce liver and kidney, strongbones and muscles, drop ministerial fire. We introduce programs for thedevelopment of Large Yin-Nourishing Pill, it contains preparation, quality criteria,stability, safety, effectivity, acute toxicity testing, long-term toxicity testing,pharmacokinetics and so on.