Experimental Research Of Wuji-Baifeng Wan On The Climacteric Syndrome Of Sleep And Hormone Levels In Tested Mice

ObjectiveClimacteric Syndrome (CS) is harmful to menopausal women's health, also is one of major diseases affecting daily life of menopausal women. Research in pathogenesis of CS is merely related to dysfunction of nervous system, endocrine system and immune system. The concept of holism of Traditional Chinese Medicine (TCM) is consistent with NEI-N theory studied by Basedovsky in 1997, the view made up a complete network among euro-endocrine-immune System. Focus on CS treatment, widely using hormone replacement therapy in modern medicine, for long-term medication and obvious side-effects, finally limits its clinical application area. Otherwise, curative therapy of TCM had its clinical purposes even reduced the damage on symptoms of CS, especially related to treatment deficiency of the kidney, based on holistic theory of TCM. Wuji-Baifeng Wan is a common gynecological medicine for menstrual disorders caused by deficiency of the kidney and it has good function. Through studying the impact of Wuji-Baifeng Wan on CS mice's sleep and related hormone, to explore the mechanism of action of Wuji-Baifeng Wan in the treatment for CS, to provide experimental evidence for the treatment of Wuji-Baifeng Wan in CS, as well as provide a theoretical basis for expanding the use of Wuji-Baifeng Wan in clinical application. Methods1. Preparation of the CS mice2. Grouping CS mice and method of drug administration3. The impact of Wuji-Baifeng Wan on the sleep of CS in mice4. The impact of Wuji-Baifeng Wan on the related hormone of CS 5. Statistical Method:data analysis of SPSS16.0 software packageResults1. The impact of Wuji-Baifeng Wan on the sleep of CS in mice, after injection of sodium pentobarbital blank control group, low, medium and high dose groups of mice of sleep occurred, respectively:3,9,9,11, the difference between high-dose group with blank control group was significant (P<0.05).2. Different doses of Wuji-Baifeng Wan can increase the serum E2levels of CS mice, medium and high dose groups control group, the differences were significant (P<0.05).3. Different doses of Wuji-Baifeng Wan can increase the serum T levels of CS mice, medium and high dose groups control group, the differences were significant (P<0.05).4. Different doses of Wuji-Baifeng Wan can reduce the serum LH levels of CS mice, medium and high dose groups blank control group, the differences were significant (P<0.05).5. Different doses of Wuji-Baifeng Wan can reduce the serum FSH levels of CS mice, the difference between high-dose group and control group was significant (P<0.05).6. Different doses of Wuji-Baifeng Wan can reduce the serum T/E2 levels of CS mice, low, medium and high dose groups and control group, the differences were significant (P<0.05).ConclusionDifferent doses of Wuji-Baifeng Wan in tested mice of CS can improve sleep in various degrees, proved that adjust the balance of yin and yang between viscera can promote the quality of sleep of CS.Kidney deficiency is closely related to hypothalamus-pituitary-ovarian (HPO) axis dysfunction which disorder of a series of related hormone and then the balance has was destroyed. Therefore, the action of Wuji-Baifeng Wan can restore the balance of relative HPO axis, and adjust the disorder of hormone levels, one by replenishing kidney essence, others through increasing serum T and E2 levels, meanwhile, reducing serum LH and FSH levels. T/E2 ratio of the CS mice can be decreased by Wuji-Baifeng Wan, suggesting that it may have a delaying effect of hypo-ovarian.Further research need to be done that Wuji-Baifeng Wan is able to adjust the levels of other hormones, or have an impact on other aspects such as immune, free radicals or adrenal gland.