Orthogonal Experimental Study Of Metal Ions And Phosphatidylglycerol Single Molecule Physical Factors Electrostatic Interactions Between Membrane

The membrane meterials of high Property is particularly important with the development of modern biotechnology. The Langmuir-Blodgett film is the most commonly used tools to study single molecule membrane, it provides important technical support for the new research methods of single molecule membrane. Metal ions is the indispensable element in the life activities, it can be to adjust the structure of the cell membrane, the pH environment of the cells activity and the osmotic pressure of balancing the cell inside and outside. Therefore, that has an important position in the field of biological membrane. Metal ions effect the life activities by the electrostatic or ligand interacts with single molecule membrane. However, the research of interaction between of metal ions and the molecule membrane is very rare and there is no universal rule. This article to mainly futher explore the law of interaction between molecule membranes. We change the external factors to study the effect of membrane. Property and find the most suitable conditions of membrane formation base on interaction between of metal ions and the molecule membrane. The research has a great potential application value in the medical field and has been widely concerned by scientists.In this paper, the first chapter is mainly introduced the membrane of the origin, development and application. The second chapter is mainly the electrostatic interactions of metal ions and molecule membrane. First, we can build the point charge model base on the material the phosphatidylglycerol molecule structure characteristics of amphiphilic about membrane formation. Secondly, we take advantage of electromagnetic theory to analysis various interactions between ions this model. Finally, we analysis and study the ion stress state in the pressure membrane. The third chapter mainly introduced the advantage of the Langmuir-Blodgett film,the material requirements, preparation and experimental apparatus. The fourth chapter is the most important part in this article, that mainly validate the theoretical analysis by the experiment. We focus on the research effect of cholesterol, the pH value and the amount of membrane formation to the phosphatidylglycerol single molecule menbrane. Finally, we introduces the validation about orthogonal experiment method and changing conditions to transfer into the formation of the Langmuir-Blodgett film. In this paper, we were studied by the theoretical analysis, the control variables, contrast analysis and the experiments can conclude:The first, the phosphatidylglycerol π-A cure which is formed by the single molecule membrane was changed more obvious with the amount of cholesterol under the same solution phase and the same amount of membrane forming substances. The second, the phosphatidylglycerol π-A cure which is formed by the single molecule membrane was changed more obvious with the increased gradually of the metal ions valence under the same amount of membrane forming substances and the same amount of cholesterol. The third, we conclude the optimal membrane forming conditions of the phosphatidylglycerol monomolecular: MgCl2for the phase, the30μL for the amount of the phosphatidylglycerol, the6.8for the pH value, the20μL for the amount of cholesterol. At the same time, we can get the influence of the phosphatidylglycerol monomolecular membrane quality:B of the highly factor, D of the significant, A of the less significant and C of the not significant. The fourth, we found the phenomenon of promotion and gathered on membrane cholesterol arrangement by the atomic force microscope. The fifth, we appropriate verified the result of the theoretical analysis and further showed that the rationality and accuracy of the theoretical analysis.