Rat Acute Soft Tissue Contusion Influenced PGE2 And Organizations Of Four Cyperus Scattered

Objective:Establishment of SD rats with acute closed soft tissue contusion model. Using the hospital late famous martial Medical expert, Professor Zheng Huaixian, clinical experience drug that is Sizhixiangfu San. Qili San come from Beijing Tong Ren Tang contrasted to Sizhixiangfu San. We had observed that rats Limbs recovery, and detected PGE2, and analyzed anti-inflammatory and analgesic effect, and provided a theoretical reference of the clinical treatment of acute soft tissue injury.Methods:72-two-month-old SD rats modeling after72rats were weighed and recorded, randomly divided into three groups(n=72), the experimental group(Cheng system the Sizhixiangfu San), drug control group(Beijing Tong Ren Tang Qili San),blank control group. Then randomly divided the three groups into four time points group(1-day group,3-day group,6-day group,10-day group, n=6). We had observed the treatment of soft tissue damage index in each of the four time points, and detected the damaged tissue content of PGE2.Results:(1)contusion symptomatology indicators:modeling damage index score of1-day, three groups of rats did not differ significantly (P>0.05), balanced comparable among the three goups; the six day, Beijing Tong Ren Tang Qili San group and blank control group have a significant difference(P<0.05), and the experimental group show no significant difference; the ten day, experimental group had significant difference with blank control group(P<0.05), and drug control group had no significant difference with blank control group(P>0.05).(2) histological observation:1,3,6days after modeling, morphological examination of the three groups of rats were no clear difference; the ten day, experimental group and drug control group were better than blank group.(3) PGE2Measuring:after treatment, the experimental group damage local tissue PGE2content gradual reduce, compared to the first day, in3-day,6-day,10-day several time points, damage local tissue PGE2had downward trend. And the1-day, the damaged local tissue PGE2content had high significant difference with later three time points(P<0.01), then the later three time points, damage local tissue PGE2content compared with each other, were no significant difference(P>0.05); the drug control group damage local tissue PGE2had downward trend too. The first day the damaged local tissue PGE2content compared to the3-day,10-day had significant difference(P<0.05). But there was no significant difference between the later three time points(P>0.05). The blank group damage local tissue PGE2content also gradual reduce, and had downward trend. But the ten day damage local tissue PGE2content compare to the fist day had significant difference(P<0.05), and compare to the six day damage local tissue PGE2content had no significant difference(P>0.05). Different group comparison:the one day, three day, six day, these three time points damage local tissue PGE2content had no significant difference(P>0.05). In10-day, the experiment group damage local tissue PGE2content compared to the blank group had significant difference(P<0.05).Conclusion:In10-day, the experiment group had clear therapeutic effect on acute soft tissue injury. Cheng system Sizhixiangfu San can shorten the repair process of soft tissue injury, and alleviate damage local pain, and can obviously reduce the damage local tissue PGE2content.