Analysis And Pharmacological Efficacy Studies Monosaccharide Composition Of Polysaccharide In Northern Shaanxi Beach Date

This study was designed to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Zizyphus jujube cv. Shaanbeitanzao (ZSP), and explore its protective effects on insulin resistance and dyslipidemia in mice. HPLC analysis showed that ZSP was the heteropolysaccharides with L-arabinose being the main component monosaccharide (50.2%, molar percentage). ZSP displayed strong antioxidant activity in vitro, and the effect was further verified by suppressing CCl4-induced oxidative stress in liver at three tested doses of ZSP (100,200, and400mg/kg·BW) in mice. Administration of ZSP (400mg/kg) significantly (p<0.01) reduced the activities of CCl4-elevated alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactic dehydrogenase (LDH) in serum, and hepatic malondialdehyde (MDA) level. Mice treated with ZSP showed a better profile of hepatosomatic index (HI) and antioxidant system with normal glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activities in liver. Besides, mice fed20%fructose in drinking water for4weeks displayed hyperglycemia, hyperinsulinemia, dyslipidemia and impaired insulin sensitivity (p<0.05). The oral administration of ZSP at400mg/kg’BW not only brought back the serum glucose, insulin and lipids profile towards normal, but also markedly improved homeostasis model assessment for insulin resistance (HOMA-IR) and p-cell function (HOMA-p).These results suggest that ZSP exerts an effective protection against CCl4-induced hepatic injury by mediating antioxidative and free radical scavenging activities. In addition, the jujube polysaccharides may ameliorate the syndromes of insulin resistance and dyslipidemia in fructose-fed mice.