Compound Of PNS Liposomal Gel Skin Dosage Forms

We choose Saponins of Panax Notoginseng (PNS) andβ-ecdysone (EDS) as the model drug and the objective of this study was to develop a transdermal delivery system——liposomal gel which could increase the drug cumulative amount in the skin. As a result, the anti-aging effect of the model drug enhanced.HPLC methods were developed for the assay of Saponins of Panax Notoginseng (PNS) andβ-ecdysone (EDS) in drug and the formulation. Film-dispersion method was developed to prepare PNS/EDS compound liposomes, with lecithin as lipid carrier and cholesterin as surfactant. The compound liposome formulation was optimized by the orthogonal experimental design and Carbomer was added as a supportive base for the preparation of liposomal gel. The final formulation was decided by permeation tests.To compare the skin permeation of PNS compound microemulsion、ointment、liposomes、gel and liposomal gel in vitro, it was concluded that liposomal gel was a better choice for topical-use drug.To investigate the anti-aging effect of PNS/EDS compound liposomal gel on skin. The aging mice model were established by subcutaneous administration with a dose of D-galactose (1000mg·kg~1·d~1) to female mice for 6 weeks, After 42 days, the SOD activity, MDA and hydroxyproline contents in skin were determined. Meanwhile, histochemical standards relating to aging were measured and morphologic alterations of surplus dorsal skin were observed under microscope and analyzed. It turned out that PNS/EDS liposomal gel has obvious anti-aging effects on aging mice’s skin.The acute toxicity and irritative test were studied. The results showed that rats were safe in acute toxicity test and Guinea pigs did not show irritative response. In conclusion, PNS/EDS liposomal gel is safe for topical administration.