| Antibiotic drug is one kind of the most widely used drugs. The microbiological method is mainly used in harmless treatment of antibiotic industrial wastewater currently. In this study, bacterial diversity of antibiotic industrial wastewater treatment system was analyzed in order to lay the foundation for further optimizing the conditions for antibiotic wastewater treatment process and screening the strains which can degrade antibiotic industrial wastewater better.In this study, the total bacterial DNA of the samples which chosen at three periods that bacteria change most were extracted respectively. V3fragments of16S rDNA of bacteria were obtained by PCR and then analyzed by denaturing gradient gel electrophoresis (DGGE). The characteristic bands and common bands were sent for sequencing. Then the results of sequencing were submitted for blast in GenBank of NCBI in order to obtain the information of sequences. At the same time, the abundance, dominance, optical density of DGGE profiles were analyzed. The results showed that the rich bacterial diversity appeared in aeration stage of SBR process, and the number of bands in the aeration stage was more abundant than precipitation and idle stage. Some bacteria in the system had maintained dominance in three stages, and some had dominance only in a single reaction time while not in other stages, and even did not appear bands. The characteristics of the SBR treatment process were faithfully reflected from DGGE profiles.It was showed that the fifteen bands had nine aerobic bacteria, such as Flavobacterium sp., Benzene decomposing sp., Frigoribacterium sp., Desulfobacterium anilini, Chryseobacterium sp., actinobacterium, Paracoccus sp., Iron-reducing sp., Acidobacteria sp.. Bacillus subtilis, and two obligate anaerobes species, Chloroflexi sp..Six of them were uncultured bacteria. The different cyclical changes of dominant bacteria were showed in different reaction time periods. |
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