Study On Fomesafen-degrading Enzyme And Soil Remediation Offomesafen-degrading Bacteria

In recent years, environmental pollution and safety issues are increasingly becoming the focus of attention, how to effectively avoid pollutants to the environment and damage to human health has become an urgent problem that needs to be solved. As an agricultural country and one of Diphenyl ether herbicides, Fomesafen has been exploited and used to enhance the development of pesticide and the agriculture production. But widespread use of Fomesafen will result in immense potential jeopardize to the ecological environment and human’s life. Therefore, removal of Fomesafen residues in soil and elimination of residual effects on succession crops has great theoretical and practical significance.In this study, by using UV spectrophotometry of enzyme activity to locate the strain F12 Fomesafen-degrading enzyme and study its enzymatic properties. High performance liquid chromatography method is used to evaluate the influence of the strain F12 degrade Fomesafen in soil under different culture conditions. Using pot experiment bioassay to conduct parallel analysis its repairing effect.The results showed that the Fomesafen-degrading enzyme of the strain F12 was ectoenzyme. The optimum conditions for the enzyme were as follows: reaction time was 30 min, enzyme concentration was 1 mL, pH was 7.5, temperature was 35℃ and the concentration of Fomesafen was 150 mg/L.Addition of the strain F12 in soil could promote the degradation of Fomesafen. The optimal conditions of the strain F12 to degrade Fomesafen were as follows: The optimal amount of the stain F12 in soil was 0.3×109 CFU/g, the initial Fomesafen concentration was 100 mg/kg, water content in soil was 20% and black soil. The degradation efficiency was 79.5% for 30 d in sterilized soil.Pot experiment showed that: the strain F12 could effectively increase the degradation rate of Fomesafen in contaminated soil and remove phytotoxicity. Under the conditions of this lab: black soil, the concentration of the strain F12 was 0.3×109 CFU/g, the initial Fomesafen concentration was 100 mg/kg, keep soil moisture content at 20%, the growth and development of wheat were in good conditions, which reached an ideal repair effect.