In Vivo Distribution And Biocompatibility Of Polyphospholipid Modified Nanodiamond And Highly Soluble Peg-coated Boron Nitride In Mice

In this article, Nanodiamond(ND) was surface modification with a hydrophilic and biocompatible polymer through ATRP(Atom Transfer Radical Polymerization) using 2-methacryloyloxyethyl phosphorylcholine(MPC) as the monomer. The successful preparation of ND-MPCpolymer nanocomposites were characterized by a number of characterization techniques. The distribution behavior as well as toxicity profiles of these ND polymer nanocomposites were also evaluated through radiolabeling technique and biological evaluation in detail. We demonstrated that the ND-MPC nanocomposites showed great improved dispersibility in aqueous solution. More importantly, ND-MPC nanocomposites showed almost no toxicity in vitro and in vivo. Furthermore, due to the good applicapability of ATRP, many other ND based polymer nanocomposites with controlled properties and functions can also be fabricated through the similar method.We first synthesized the highly solubility boron nitride(BN) nanoparticles coated by Polyethylene glycol(PEG) with smaller size(~10 nm), then studied their biodistribution in vivo through radioisotope(Tc99mO4-) labeling, and the results showed that BN-PEG nanoparticles mainly accumulated in the liver, lung and spleen with the less uptake by brain. Moreover, the pathological changes induced by BN-PEG could be significantly observed in the sections of liver, lung, spleen and heart, which can be also supported by the test of biochemical indexes in serum. More importantly, we first observed the biodistribution of BN-PEG in the heart tissues with high toxicity, which would give a warning about the cardiovascular disease, and provide some opportunities for the drug delivery and treatment.