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Study On The Mating System And Morphological Development And Breeding Of Hericium Coralloides

Posted on:2017-02-08Degree:MasterType:Thesis
Country:ChinaCandidate:X HuFull Text:PDF
GTID:2283330503466189Subject:Vegetable science
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Hericium coralloides form spectacular coral in the sea, fruiting bodies of tender ment. Sweet natured,with good nutrition and health value and with a nourishing,aid digestion and other effects. Since the history of cultivation short of Hericium coralloides, large-scale artificial cultivation is not yet implemented, and the production process in the presence of slow growth, hyphae thin and so on.In the process of growth and development growth,this experiment aims at first fungus strains which suited the hypha growth temperature and appropriate growth p H value,and proposes the corresponding cultivation techniques procedures.In recent years, For.Hericiumcoralloides.mating system research,until now,there is no relevant report at homme ad abroad In this paper, excellent strains were bred by single- single hybridization methods. Through structural botany test methods, the morphological development of Hericium coralloides were studied, providing the basis for resource development and utilization of Hericium coralloides1、Studying on breeding of Hericium coralloidesHericium coralloides is intermediate temperature constant temperature of Fecundity fungus. Mycelium optimum growth temperature is 25℃, mycelial growth in the range of 5-30℃.Numbers of good or bad Hericium coralloides mycelium and nitrogen are closely related, excess nitrogen, mycelium leggy, nitrogen deficiency, thin hyphae. PDA medium, 0.5% peptone, dense mycelial growth, substrate mycelium more. Mycelium in the range p H4-9 are able to grow, the optimum p H of 5.2、Mating system of Hericium coralloidesWith an microscope and fluorescence microscope method of combining, We collect a total of 27 single strain identification.By there rounds mating method,it is determined that the mating system of Hericium coralloides is four, and four kinds of mating type to 1:1:1:1segregation rule.3、Studying on morphological development of Hericium coralloidesIn this study, collecting basidiospores, spore shape, spore germination, mycelium primary, secondary mycelium, primordium, Gulei, various stages of fruiting body of research of Hericium coralloides, which describe the morphological changes during growth and development, a clear morphological development rule of Hericium coralloides. This spores colorless, nearly spherical, high transparency; In PDA liquid medium, the basidiospores swelling at 24 h, at 36 h formation of germ tube, at 84 h germination generate primary hyphae;Primary hyphae branch small and thin, have not clamp connection; more secondary hyphae branch, with a clamp connection. Primordia differentiation generate first main branch of Hericium coralloides, followed by gradual lateral branches, fungus thorn finally mature to form a complete fruiting bodies.4、Breeding of new varietiesIn this experiment,excellent hybrid strain by mating system produced of Hericium coralloidest,together produce hybrid combinations 19, through temperate and p H testing, screening mycelium growth stage of fruiting excellent 10 are tested by agronomic traits, that yield screened a high yield strains 144-145...
Keywords/Search Tags:Hericium coralloides, basidiospore, hyphae morphology, mating type, breeding fine strain
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