Study On The Anti-browning Drying Technology And Quality Of Lili Bulbus

ObjectiveOptimizing anti-browning drynesses and solving the browning problem, in order to establish a set of Anti-browning drying technology with the advantages of safety simple and general practicality. To set up determination methods of the main effective components and SO2. Detecting the phenolic acids for the sake of improving the quality standard of Lili bulbus.Methods(1) Adopted bubbling, moisture content browning degree, yellowness index and total polyphenol as indicators optimal drying technology parameters of Far-Infrared hot air dryness, Electric hot air dryness, Vacuum-heating dryness and Microwave-heating dryness by orthogonal design and synthetic weighted method.(2) Using Folin—Ciocaileu, Phenol-sulphuric acid, Molybdenum blue, perchloric acid and pararosaniline hydrochloride colorimetry detect total polypheno, total polysaccharide, total phospholipids, total saponins and SO2. Comparison on the content of main effective components from different drynesses and Longshan county.(3) Establishing the high performance liquid chromatographic(HPLC) fingerprints and primary identifying compounds of the phenolic by HPLC and HPLC-MS. Chromatography conditions as follows:an ODS C18 column(250 mm×4.6 mm,5μ.m) was employed, the gradient elution of acetonitrile and 0.1%formic acid was used as mobile phase with a flow rate of 1mL·min-1, the detection wavelength was set at 315 nm and the column temperature was set at 25℃.Results(1) The optimized drying technology conditions as follows: Far-Infrared hot air dryness:the initial drying temperature is 115℃, the tail temperature is 85℃; the initial drying time is 70 min, the tail drying time is 50 min; the material thickness is 1.5cm. Electric hot air dryness:the initial drying temperature is 115℃, the tail temperature is 85℃; the initial drying time is 55 min, the tail drying time is 65 min; the material thickness is 1.0cm. Vacuum-heating dryness:the drying temperature is 70℃, the drying time is 14h, the material thickness is 1.0cm. Microwave-heating dryness:the microwave power is 3.40kw, the drying temperature is 60℃, the drying time is 10min, the material thickness is 1.0cm.(2) Established a set of simple and rapid methods for determining the main effective components in Lili bulbus.(3) The high performance liquid chromatographic (HPLC) fingerprints of the phenolic was established. Detecting fingerprints of 10 batches of different drying technologies. The results showed that there were 12 common peaks contained in the tested samples. Primary identified the phenolic acids by HPLC-MS and references.Conclusion(1) The Anti-browning drying technologies and conditions of Lili bulbus are Optimized. Based on effective components and browning degree factors to comprehensive assessment of the different Anti-browning drying technologies, the best one is Far-Infrared hot air dryness, the second one is Electric hot air dryness, the third one is vacuum-heating dryness, and the last one is Microwave-heating dryness. The quality of those Products are better than dried lily bulbs from Longshan county.(2) Determining the main effective components of total polysaccharide, total saponins and total phospholipids, It provides reference about improving quality standard of Lili bulbus.(3) The HPLC fingerprints of phenolic acids in Lili bulbus show good characteristic and repeatability, and the method is stable and reliable, it can be used for the study of browning material basis and mechanism in Lili bulbus.