Effects Of Food Ingredient Group On Oxidative Stresses And Trace Elements In Male Mices Exposed To Lead

Objective Analysis of food ingredient group on lead poisoning mice whole blood，liver，kidney，brain tissue antioxidant enzymes and trace elements influence.Methods Ninety18-20g Kun-Ming male mice were randomly divided into following6groups: the negative control group; the lead acetate model group; the positive drug groupand the test substance groups with low, medium and high dose food ingredient group. Inaddition to the negative control group, other groups were provided with drink containing1.00g/L lead acetate solution. After30days, the test substances were oral gavage fed with thefood ingredient group in with the doses of3,6and12g/(kg b.w.*day) during30daysrespectively, which were equivalent of5,10and20times of the dose0.60g/(kg b.w.*day)recommended for human. The positive drug group were gavages’ fed with dimercaptosuccinicacid solution(EDTA). The lead acetate model group and the negative control group weregavages’ fed with deionised water. One month later，determintion of SOD，GSH-PX，MDAand Pb，Ca，Fe，Cu，Zn content in whole blood，liver，kidney and brain tissue.Result the lead acetate model group mice whole blood，liver，kidney，brain tissue Pblevels were significantly higher than the negative control group(P<0.05). Comparing with thelead acetate model group，in addition to the low dose food ingredient group displayed nostatistics differences in kidney Pb level(P>0.05).Other test substance groups and the positivedrug group whole blood，liver，kidney，brain tissue Pb levels were significantly lower than thelead acetate model group(P<0.05).Comparing with the negative control group， the lead acetate model group whole blood，liver，kidney and brain tissue showed significantly lower SOD，GSH-PX levels (P<0.05) and significantly higher MDA levels (P<0.05). Comparing with the lead acetate model group，themedium and high dose groups whole blood， liver， kidney and brain tissue showedsignificantly higher SOD，GSH-PX levels (P<0.05) and significantly lower MDA levels(P<0.05). Comparing with the positive drug group，the medium and high dose groups wholeblood and brain tissue showed significantly higher GSH-PX levels，liver and kidney tissueshowed significantly lower SOD levels(P<0.05)；the high dose group whole blood and kidneytissue showed significantly lower MDA levels，and kidney tissue showed significantly higherGSH-PX levels(P<0.05)；the medium dose group liver tissue showed significantly higherGSH-PX levels and kidney tissue showed significantly lower MDA levels(P<0.05)；Othercomparative differences are not statistical significance(P>0.05).Comparing with the negative control group，the lead acetate model group whole bloodshowed significantly higher Ca，Cu levels and lower Fe，Zn levels(P<0.05)；the lead acetatemodel group liver tissue showed significantly higher Zn levels and lower Fe， Culevels(P<0.05)；the lead acetate model group kidney tissue showed significantly higherCa，Zn levels and lower Fe，Cu levels(P<0.05)；the lead acetate model group brain tissue showedsignificantly higher Ca，Cu levels and lower Fe levels(P<0.05).Comparing with the lead acetate model group，the test substance groups and the positivedrug group whole blood showed significantly lower Ca， Cu levels and higher Felevel(P<0.05)；the medium and high dose groups whole blood showed significantly higher Znlevel(P<0.05)；the high dose group liver tissue showed significantly higher Fe level(P<0.05)；the medium，high dose groups and the positive drug group liver tissue showed significantlylower Zn level(P<0.05)；the medium and high dose groups kidney tissue showed significantlyhigher Fe level and lower Zn level(P<0.05)；the positive drug group kidney tissue showedsignificantly lower Ca level(P<0.05)；the test substance groups and the positive drug groupkidney tissue showed significantly higher Cu level(P<0.05)；the test substance groups and thepositive drug group brain tissue showed significantly lower Ca level(P<0.05)；the test substance groups brain tissue showed significantly higher Fe level(P<0.05)；the high dosegroup brain tissue showed significantly higher Zn level and lower Cu level(P<0.05).Comparing with the positive drug group，the test substance groups whole blood and braintissue showed significantly higher Fe levels(P<0.05)；the test substance groups whole bloodshowed significantly lower Ca level(P<0.05)；the high dose group whole blood showedsignificantly higher Zn level and brain tissue showed significantly lower Ca level(P<0.05)；thelow dose group liver tissue showed significantly higher Zn level and kidney tissue showedsignificantly higher Pb level(P<0.05)；；Other comparative differences are not statisticalsignificance(P>0.05).The correlstion analysis results showed the lead-exposed mices whole blood，brain tissuePb，Ca，Cu，MDA contents and excretion of food doses are negatively correlated；Fe，Zn，SOD，GSH-PX contents and excretion of food doses are positive correlated(P<0.01). thelead-exposed mices liver，kidney tissue Pb，Zn，MDA contents and excretion of food dosesare negatively correlated；Fe，SOD，GSH-PX contents and excretion of food doses are positivecorrelated(P<0.01). the lead-exposed mices kidney tissue Cu content and excretion of fooddose is positive correlated(P<0.01).Conclusion The food ingredient group has a role in promoting lead excretion andantioxidant. Lead poisoning-induced oxidative damage has a good improvement.At the sametime，The food ingredient group can regulate the body’s trace element metabolism disorders.The efficacy and excretion of food dose has a definite correlation.