Study On The Adiponectin Gene Polymorphism Between Hui Metabolic Syndrome Patients And Coronary Heart Disease

ObjectiveThe purpose of this study was to investigate the genotype distribution of adiponectin geneSNP+45T/G and SNP+276G/T polymorphisms of Ningxia Hui nationality，analyse therelationship between adiponectin gene SNP+45,SNP+276and MS of Hui，analyse therelationship between adiponectin gene SNP+45,SNP+276and CHD of Hui，Understand theinfluence factors of MS and CHD.MethodChoose normal control group (n=98), metabolic syndrome group (MS group,n=105),metabolic syndrome with coronary heart disease group (M+C group, n=102);Usingexperiment-control study, analysis of adiponectin gene SNP+45and SNP+276Polymerasewith chain reaction-restriction fragment length polymorphism (PCR-RFLP);Assay of Plasmaadiponectin concentration with Enzyme Linked Immunosorbent;Assay of fasting insulin withRadiation immune; calculating BMI use the formula: weight (kg)/height (m)2.Results1. Clinical characteristics of three groupsThere were no significant differences in age and gender between the three groups(P>0.05). Compared with controls，body mass index(BMI)，systolic blood pressure(SBP)，diastolic blood pressure(DBP)， wist-to-hip ratio (WHR), triglyeerides (TG),totalcholesterol(TC), LDL-C low-density lipoprotein cholesterol (LDL-C), fasting blood glueose(FBG) were significantly higher in MS and M+C groups (P<0.05),whereas high-densitylipoprotein cholesterol (HDL-C) was signifieantly lower in MS and M+S groups (p<0.05).Compared with MS group, FPG, TG，TC were significantly higher in M+C group (P<0.05), HDL-C was significantly lower in M+C group (p<0.05)).2. Plasma adiponectin concentration and HOMA-IR of three groupsThere were significant differences in plasma adiponectin concentration and HOMA-IRbetween the three groups （P<0.05）. Compared with controls, plasma adiponectinconcentration was significantly higher in MS and M+C groups (P<0.05), whereas HOMA-IRwas signifieantly lower in MS and M+S groups (p<0.05). Compared with MS group, plasmaadiponectin concentration was significantly higher in M+C group (P<0.05).3. Genotype distribution and allele frequencies of three groupsSNP+45site Genotype distribution and allele frequencies were significant differencesbetween the three groups (P<0.05), χ~2=105.685，P=0.000；χ~2=55.928，P=0.010（P<0.05）.Compared with controls, SNP+45TG+GG genotype distribution and G allele frequencieswere significant higher in MS group (P<0.05), χ~2=53.012，P=0.000；χ~2=51.377，P=0.000（P<0.05）. Compared with controls and MS group, SNP+45TG+GG genotypedistribution and G allele frequencies were significant higher in M+C group (P<0.05),χ~2=76.256，P=0.000,；χ~2=39.488，P=0.000；χ~2=19.544，P=0.000；χ~2=7.710，P=0.02（5P<0.05）。SNP276site Genotype distribution and allele frequencies were significant differencesbetween the three groups (P<0.05), χ~2=18.130，P=0.001；χ~2=7.384，P=0.025（P<0.05）.Compared with controls, SNP+276genotype distribution and allele frequencies were nosignificant differences in MS group, χ~2=0.002，P=0.999（P>0.05）；χ~2=0.000，P=0.989（P﹥0.05）(P<0.05). Compared with controls and MS group, SNP+276TG+TT genotypedistribution and T allele frequencies were significant higher in M+C group (P<0.05),χ~2=14,395，P=0.001；χ~2=8.829，P=0.003；χ~2=14.509，P=0.001；χ~2=15.093，P=0.010（P<0.05）.4. Logistic Regression Model of MS risk factorsStepwise multivariate logistic regression analysis was performed to determine thepredictive variables of MS among SNP+45T/G and SNP+276G/T Polymorphism, age,sex,SBP,DBP,FBG,TG,TC,HDL-C,LDL-C, BMI, HOMA-IR, plasma adiponectinconcentration,which showed that BMI, SBP, DBP,FPG,TG,HDL-C,plasma adiponectinconcentration，SNP+45T/G polymorphism were the independent predietors. Carriers with Gallele frequencies had higher risk of MS.5. The affect of adiponectin polymorphisms on the components of MSMultivariate logistic regression analysis was used to determine the predietive variables ofMS among SNP+45T/G and SNP+276G/T polymorphisms, age, sex, SBP, DBP, FBG, TG,TC, HDL-C, LDL-C, BMI,HOMA-IR, plasma adiponectin concentration，for the componentsof MS，which showed that SNP+276site G/T Polymorphisms were increased the risk of highIR. SNP+45T/G Polymorphisms were increased the risk of high FPG, TG, BMI and SBP.6. Logistic Regression Model of CHD risk factorsStepwise multivariate logistic regression analysis was performed to determine thepredictive variables of CHD among SNP+45T/G and SNP+276G/T Polymorphism, age, sex,SBP, DBP, FBG, TG, TC, HDL-C, LDL-C,BMI,HOMA-IR, plasma adiponectinconcentration,which showed that FPG, HDL-C, LDL-C,plasma adiponectin concentration，SNP+45T/G and SNP+276T/G polymorphism were the independent predietors. Carrierswith SNP+45G and SNP+276T allele frequencies had higher risk of CHD.7. Comparison of the plasma adiponectin concentration and HOMA-IR of different GenotypeCompared with SNP+45TT genotype, the plasma adiponectin concentration wassignifieantly lower in TG+GG genotype, and HOMA-IR was significantly higher in TG+GGgenotype (p<0.05). Compared with SNP+276GG genotype,the plasma adiponectinconcentration was signifieantly lower in TG+TT genotype, and HOMA-IR was significantlyhigher in TG+TT genotype (p<0.05).8. Affect factor of the plasma adiponectin concentrationMultivariate logistic regression analysis was used to determine the predietive variables of APN among SNP+45T/G and SNP+276G/T polymorphisms, age, sex, SBP, DBP, FBG, TG,TC, HDL-C, LDL-C,BMI, HOMA–IR,which showed thatSNP+45T/G Polymorphisms,SBP,HOM-IR.Conclusions1. SNP+45G alleles increased the risk of metabolic syndrome in Hui.2. The metabolic syndrome patients with SNP+45G、SNP+276T alleles increased the riskof coronary heart disease.3. The patients of Ningxia hui metabolic syndrome and coronary heart disease have lowplasma adiponectin level4. SNP+45G、SNP+276T alleles of iNingxia hui have low plasma APN level5. SNP+45G alleles increased the risk of low plasma adiponectin level、high triglycerides、high systolic blood pressure、fasting blood-glucose、obesity, SNP+276T alleles increaseinsulin resistance increasing risk.