Role Of Heme Oxygenase-1Expression In Cirrhotic Rats

Objective To develop an ideal means to induce rat’s liver cirrhosis and endoxtoxemicby individuation of Thioacetamide(TAA) dosage based on rat’s variations in response toTAA.Further reaearch of heme oxygenase-1(HO-1)for the TAA SD rats cirrhosis modelpathophysiological change influence,in order to investigate the liver tissue HO-1expressionon experimental liver cirrhosis endotoxin blood disease rat significance.Method Healthy male SD rats80just random into three groups,group1(20only)give clear water for drinking water,as control group,the group;2(30only)to0.03%TAA asdrinking water,group;3(30only) to0.03%TAA as the initial concentration,according to theweight of drinking water a week adjustment TAA concentration.Dose of rats induced byindividual liver cirrhosis,liver cirrhosis model induced time12weeks.Furthe the rat models ofliver cirrhosis and healthy rats are divided into four groups:1.The control groupnormal+LPS;2.Livercirrhosis+saline(TAAgroup);3.Livercirrhosis+LPS(TAA+LPS);4.Livercirrhosis+HM+LPS(HM group);Conventional biochemical method in the plasma usable aminoacids shift enzyme(AST),Alanine amino shift enzyme(ALT), nitrate reductase plasma NOconcentration measure ment method.Immunohistochemical method to detect liver tissueHypersensitivity two-step HO-1expression and distribution,use computer image analysissoftware to HO-1expression quantitative analysis.Result Individual induction dose in the rat model2liver cirrhosis rats, mortality was27%(8/30), Cirrhosis formation rate of only55%(16/30), Three group, mortality is0, livercirrhosis formation rate90%(27/30), The control group all survived.2. HO-1expressioncontrol group、TAA、TAA+LPS、HM each group of rats grey-scale values obvious reduced inturn. Each group are statistically significant differences between.3. Control group、TAA、TAA+LPS、HM Each group of plasma NO, ALT, AST in turn increases, And each group have statistically significant differences between.Conclusion1. According to the process of mould made large rat TAA reaction ofindividual differences, adjust the TAA induction dose, This method can be significantlyreduced mortality rats, improve the success rate of liver cirrhosis made mode;2. HO-1throughits oxidative damage and promote fibrosis mechanism to promote rat liver cirrhosis endotoxinblood disease lier damage.