Association Between CXCL16Gene A181V Polymorphism And TOAST Classification

Objective To investigate the relationship between CXCL16gene A181V polymorphism, serum CXCL16levels and cerebral infarction subtypes according to TOAST.Methods215patients with cerebral infarction and184healthy controls were enrolled in this trial. The polymorphisms of CXCL16gene A181V were analyzed by polymerase chain reaction—restriction fragment length polymorphism (PCR-RFLP). The serum CXCL16levels in74healthy controls and177patients with acute cerebral infarction were measured by Enzyme linked immunosorbent assay (ELISA).Results The serum CXCL16levels of patients were significant higher than those of healthy controls (P<0.01). After grouped the patient controls according to TOAST we found that, serum CXCL16levels were significant different among large artery atherosclerotic stroke (LAA) group, small artery occlusion stroke (SAO) group and healthy controls (P<0.01). Logistic regression revealed that serum CXCL16levels were an independent risk factor for cerebral infarction. The CXCL16gene A181V genotype distribution and allele frequencies showed no differences between patient controls and healthy controls (P>0.05). However, between LAA group, SAO group and healthy controls the AA homozygotes had significantly higher cerebral infarction risk compared with the G allele carriers (GA+GG)(P<0.05). CXCL16gene A181V polymorphism was associated to serum CXCL16levels (P<0.05)Conclusion High serum CXCL16level is a risk factor for cerebral infarction. Serum CXCL16level is affected by CXCL16gene A181V polymorphism. A allele may be useful to identify patients at increased risk of atherosclerosis.