Research On Quality And Fingerprint Of Cultured Cordyceps Militaris

Cordyceps militaris, also known as Cordyceps, North Cordyceps. Cordyceps militaris is an edible and medicinal fungi which was recognized at home and abroad,.It is in favor of Lungs and kidney, and has function include anti-fatigue, hypoxia tolerance, regulate immune, and auxiliary anti-tumor effects. The main active composition of Cordyceps militaris including cordycepin acid, Cordyceps polysaccharide, is also rich in vitamins and amino acids, as well as a variety of essential microelement. because easy to large-scale artificial cultivate and similar to Cordyceps sinensis on medicinal ingredients, Cordyceps militaris to be a superior alternative to Cordyceps sinensis, can be solved the situation of scarcity of of wild Cordyceps sinensis resources on some extent. Cultured cordyceps militaris cultivation techniques to enter the industrial production stage, But the quality of Cultured Cordyceps militaris on the market is uneven, and even often counterfeiting. Chinese pharmacopoeia has not received contained about Cultured Cordyceps militaris quality control standards. Therefore, the establishment of Cultured Cordyceps militaris quality standards, control the quality of Cultured Cordyceps militaris, standardize Cultured Cordyceps militaris mode of production is imperative. In this study, the intrinsic quality of the10batches of cultured Cordyceps militaris was invest comprehensively and systematicly, and establish a scientific、reasonable and feasible quality standards. The main content is as follows:1. Pharmacognosy identification of cultured Cordyceps militarisOn the guide of Chinese Pharmacopoeia (2010edition), Cultured Cordyceps militaris on traits observed, microscopic identification, moisture, total ash, acid insoluble ash, water-soluble extract,and the heavy metal content were determined, Cultured Cordyceps militaris inherent quality were invested and content limits about these indicators were made according to those measurement results, provisions.2. Determination of Cultured Cordyceps militaris Polysaccharide contentCultured Cordyceps militaris medicine in the10batches of polysaccharide content were determined by the anthrone-sulfuric acid chromatometry, The results demonstrated that content discrepancy among10batches of Cultured Cordyceps militaris polysaccharide was very significant, indicating that the polysaccharide content was affected by a variety of factors in the growth of Cultured Cordyceps militaris obviously, not was included in the quality standard draft.3. Determination of the contents of the four nucleoside substances in cultured Cordyceps militarisThe contents of four nucleosides substances (cordycepin, adenosine, adenine, uridine) in10batches of Cultured Cordyceps militaris were determined by HPLC, a fast, simple, stable method that simultaneousiy determined four kinds of nucleoside substances was established. The results showed that discrepancy among10batches of Cultured Cordyceps militaris four substances was significant, cordycepin and adenosine contents are higher, content limits were made about four nucleoside of Cultured Cordyceps militaris according to the measurement results.4. Establishment of HPLC fingerprint of cultured Cordyceps militarisFingerprint of Cultured Cordyceps militaris by HPLC were established. the sample solution extraction, chromatographic conditions were investigated and optimized.the chromatogram obtained stable baseline, well response value and separation, conformed the technical requirements of the Traditional Chinese Medicine Fingerprint. The fingerprint consist of14common peak.The similarity coefficients between every Cultured Cordyceps militaris sample and the control fingerprint were exceeded0.9.5. The establishment of quality standards of cultured Cordyceps militarisDrafted cultured Cordyceps militaris quality standards on the basis of all experimental studies, provide a scientific basis to evaluate the quality and be received by Pharmacopoeia of cultured Cordyceps militaris.