The Protective Effect Of Lactones Ginkgo Biloba Injection On Focal Cerebral Ischemia In Rats

Objective: To investigate the protective effect of lactones ginkgo biloba injection onfocal cerebral ischemia in rats, and analyze the possible mechanism.Methods:60SPF healthy male Sprague-Dawley rats were randomly allocated into fivegroups(n=12):(1) the sham operation group,(2) the model group,(3) the pre-treatmentgroup,(4)2hours of medicine group after the surgery，(5)6hours of medicine groupafter the surgery. Injected in the intraperitoneal according to body surface area oflactones ginkgo biloba injected17mg/kg; the pre-treatment group were injected withlactones ginkgo biloba injection17mg/kg on1hour before operation, while the shamoperation group and model group were given equal volume of saline. The ischemic modelwas established with line embolism to block the middle cerebral artery. We measured thescore of neurological deficits after24h of ischemia. Then all the rats were killed to obtainthe brain tissues. We calculated infarct volume by2，3，5-triphenyl tetrazolium chloride(TTC) staining to measured brain containing water and the cerebral infarction areaproportion. The content of caspase-3and cytochrome C in the rat cerebral tissue weredetermined with Enzyme linked immunosorbent (ELISA).Results:①Comparedwith the sham group, model group, the pre-treatment group,2hours to medicine group after the surgery,6hours to medicine group after the surgery, ratsneurological score was significantly higher and large area cerebral infarction. Comparedwith the model group， pre-treatment group,2hours to medicine group after the surgeryand6hours to medicine group after the surgery, neurological deficit scores could bereduced (P <0.05). However, compared with pre-treatment group，2hours to medicinegroup after the surgery,6hours to medicine group after the surgery, there were nosignificant differences on neurological function scores.②Compared with model group,the pre-treatment group,2hours to medicine group after the surgery,6hours to medicinegroup after the surgery, brain water content and infarct size could be reduced (P <0.05). Tocompare Pre-treatment group with2hours to medicine group after the surgery,6hours tomedicine group after the surgery，there were significant differences in the brain watercontent and cerebral infarction area(P <0.05). Compared with the pre-treatment group,2 hours to medicine group after the surgery,6hours to medicine group after the surgery,cerebral edema and infarct size were increased. The effect in the pre-treatment group wasbetter. Compared with2hours to medicine group after the surgery and6hours to medicinegroup after the surgery, there were no statistically significant differences in brain watercontent.③Compared with the sham group, the model group, the pre-treatment group,2hours to medicine group after the surgery,6hours to medicine group after the surgery, thecontent of cytochrome C in rat brain cortex was raised and there were significantlydifferences (P=0.00). Compared with model group, the pre-treatment group,2hours tomedicine group after the surgery,6hours to medicine group after the surgery, the contentof cytochrome C in rat brain cortex was reduced and there were significantly differences (P=0.00). Compared with the pre-treatment group,2hours to medicine group after thesurgery,6hours to medicine group after the surgery，the content of cytochrome C in ratbrain cortex was increased and there were significant differences (P <0.05)④Comparedwith the sham group, model group, the pre-treatment group,2hours to medicine groupafter the surgery,6hours to medicine group after the surgery, the content of caspase-3inthe rat brain cortex was enhanced and there were significant differences (P <005.).Compared with the model group, the pre-treatment group,2hours to medicine groupafter the surgery,6hours to medicine group after the surgery, the content of caspase-3was reduced. Compared with pre-treatment group,2hours to medicine group after thesurgery,6hours to medicine group after the surgery,the content of caspase-3wasincreased and there were significant differences (P <0.05). Compared with2hours tomedicine group after the surgery and6hours to medicine group after the surgery, therewere no significantly differences in the content of caspase-3.Conclusion: Lactones ginkgo biloba injection has protective effect on focal cerebralischemia. It can reduce the change in cerebral edema, brain infarction area, thecytochrome C and caspase-3content of rat brain cortex. The content of caspase-3andcytochrome C is reduced，cell apoptosis is restrained. Perhaps this is one of theprotective mechanisms.