The Expression Level Of MicroRNA218in Gliomas And Its Effects On Glioma Cell Invasion And Migration

Background and purpose：In clinical, brain tumor accounts for90%of all central nervous system. Gliomasare the most common type of malignant primary brain tumor. Gliomas arecharacterized by high invasion, migration and proliferation abilities. However, themolecular mechanism that triggering the development and recurrence of this tumor isalso elusive. Thus, the treatment for the method or drugs of tumor are also limited,and most patients diagnosed with gliomas median survival less than one yearEspecially for the glioblastoma multiforme, with the more lower survival rat inclinical. Therefore, it’s very necessary to explore the mechanism of the invasion andmigration of the glioma, and discover the more effective therapeutic methods or drugs.MicroRNAs are a group of small, non-coding18to25nucleotide-long RNAs thatplay important roles in cancer development by regulating the activities of specificmRNA targets.According to the previous studies, appropriate60%of human genes areregulated through translational repression and/or mRNA decay by microRAN.MicroRNAs indicated that microRNAs have a very close associations with gliomadevelopment.ccording to the functions in the tumor development, the microRNAcould be subdivided into two groups, including oncogenic microRNAs andtumor-suppressor microRNAs. MicroRNA218acts as a tumor-suppressor microRNAin human tumors. Recent years, microRNA218has been extensively studied iinseveral tumors,and the expression is significant decreased in human gliomatissues.However, the biology impacts of microRNA218on glioma cell invasion andmigration are also poorly understood. This study aims to investigate the expressionlevel of miR-192in human gliomas and its correlation with its different clinicalgrades of gliomas，and the biological function of microRNA218.Methods：（1）Tissues from38cases of gliomas confirmed by pathological types werecollected for Total miRNAs extracted. （2）The expression level of miR218in human gliomas tissues was quantified byreal-time polymerase chain reaction (Real-time PCR) analysis respectively and thecorrelation of the miR218expression with glioma grades was analyzed（3）The Lipofectamine2000reagent (Invitrogen, USA) was employed totransfect the the pcDNA3.1-microRNA218plasmid into U87MG and U251MG cells.（4）Glioma cell viability was detected by the CCK-8cell counting assay.（5）Transwell assays and wound-healing assay were employed to examine themigration and invasion of the glioma cells.Results：（1）MicroRNA218level negatively correlates with the gliomapathologicalgrading.（2）MicroRNA218inhibits the proliferations of U87MG and U251MG.（3）MicroRNA218inhibits U87MG cell migration and invasion in vitro.Conclusion：（1）MicroRNA218level negatively correlates with the glioma pathologicalgrading.（2）MicroRNA218could reduce the invasion and migration, and inhibitproliferation of glioma cells...