Compared Research On Clinical Visual Electrophysiological Testing System

Visual electrophysiology technology is an objective means to test the visual function,and include various testing item. Flash or pattern stimulation may evoked the variouslevels of cells electrical responses, and those biological electrical responses could reflectthe visual function. It has been widely used in clinical and animal experiments. There aremany factors that have an effect on the clinical visual electrophysiological test results,suchas the laboratory environment, electrodes, stimulators, instrumentation hardware featuresand so on.Now there is many visual electrophysiology testing system on the market.RETI-scan visual electrophysiology system (German Roland company), Diagnosis visualelectrophysiology system (U.S.A Epision company) and France Metro Vision visualelectrophysiology system are commonly used imported clinical visual electrophysiologicaltesting system in domestic. Home-made GT-2000NV visual electrophysiology system(Chongqing China), BIO-2000visual electrophysiology system (Nanchang China),IVE-205A visual electrophysiology system (Chongqing China) and APS-2000A visualelectrophysiology system (Chongqing China)also occupy a certain market share.Theoretically, the testing system should meet the requirements of the InternationalClinical Electrophysiology Visual (ISCEV) standard guidance. However, in practice, wenoticed there are some differences still between the testing system, and some are significant differences. It might bring a significant impact on clinical diagnostic analysisand experimental results if the differences between testing systems led to the misjudgmentof the results. In this paper, we compared the RETI-scan, Diagnosis, and GT-2000NVclinical visual electrophysiology system based on our previous works. Additionally, weobserved the electroretinogram (ERG) by color light evoked in normal subjective andcentral serous chorioretinopathy (CSC) patients, and in wild SD rats and retinal conedisfunction (RCD) rats. Through the research,we try to learn the differences between thevarious systems, and provide a reference for the clinical application and animal study.Materials and Methods30military youth participants (60eyes), aged20-24years old, and with no eyediseases,were randomly divided into two groups. We use the RETI-scan visualelectrophysiology systems and the Diagnosis visual electrophysiology system to recordERG, visual evoked potential (VEP) and multifocal ERG (mfERG) as similar stimulatingand recording parameter condition. Color light （red, white, green and blue）evoked ERGwere compared in normal subjective. In order to learn the feature of color ERG in wildSprague-Dawley（SD）rats retinal cone dysfunction（RCD）rats, the rat were anesthesizedafter12h dark adaptation. After the pupil were dilated, the color ERG were recorded byRETI-scan and GT-2000NV visual electrophysiology systems.Statistics were analyzed bySPSS software.Result1. There were statistically significant difference (P <0.05) in ERG, VEP and mfERGresults between RETI-scan visual electrophysiology system and Diagnosis visualelectrophysiology system recording. The color light ERG results in rats were significantdifference (P<0.05) between RETI-scan and GT-2000NV visual electrophysiology systemeither, but there were no significant difference in other testing items2. Under dark adaption, the red light evoked amplitude of ERG b wave was the lowest, and the blue light induced the highest amplitude value in normal subjectives.Under light adaption, there was no significant difference in the amplitude of b-waveevoked by red, white, green and blue color stimulation. There was no significantdifference between normal people and central serous chorioretinopathy patients in colorlight ERG.3. The ERG response of wild SD rats were exist under different color stimulation.Theb wave amplitude of Scotopic0.01ERG、Scotopic3.0ERG、Photopic3.0ERG andPhotopic3.0flicker stimulated by blue or green light is high than stimulated by white orred light,while there is no similar phenomenon in Scotopic3.0OPs.4. The color ERG in RCD rats could be recorded under dark adaptation and it issimilar to the wild SD rats, while the amplitude of b wave was lower than later. ThePhotopic ERGs stimulated by different color in RCD rats were not detected.Conclusion1. Most results of the visual electrophysiological testing items recorded byRETI-scan and Diagnosis visual electrophysiology system has significant difference (P<0.05)，Such differences might be related to the parameters of system, calculation methodsof software.Therefore, the laboratory should establish its own reference range.2. The color ERG in normal subjectives and central serous chorioretinopathypatients is similar and both were characterized by that the amplitude of b-wave are thelowest evoked by red light while the highest evoked by blue light under dark adaption.And there is no significal difference evked by different color light evoked under lightadaption. There is no significant difference in ERG wave between normal subjective andcentral serous chorioretinopathy patients.3. The results of color ERG wave in rats recorded by RETI-scan and GT-2000NVsystems have significant difference (P <0.05), but most of the testing results are relevantbetween two systems.4. The RCD rats have a similar color ERG wave to wild SD rat under dark adaptation, but the photopic ERGs could not detected.