Effect Of The Charge On The Liposomal Influenza Vaccine Immunogenicity

In order to obtain a stable, efficient flu vaccine, this paper on the basis of the neutral liposomes as adjuvant, in order to improve the immunogenicity of influenza vaccine, cationic liposomes as a vaccine adjuvant research now.This research is divided into four chapters, the first chapter for the introduction of influenza vaccine liposome overview of the system; The second chapter for flu vaccine liposome preparation and its physical and chemical properties of producing:mainly soy lecithin, cholesterol and cholesterol hydrochloride (DCCHOL) as raw material, adopts the membrane dispersion freeze-dried liposome preparation of influenza virus vaccine powder, neutral liposomes can be obtained from dry and cationic liposome, powder made of two kinds of flu vaccine liposome powder particle size, coating rate situation has carried on the comprehensive investigation; The third chapter for the charge of influenza vaccine liposome immunogenicity of animal immune experiment:the influence of further use of neutral liposomes powder and cationic liposome powder two flu vaccine liposome powder on kunming mice were respiratory immunity. Cracking vaccine (H1N1) influenza virus as immunogen, using indirect ELISA method for determination of immune serum antibody levels in mice, thus further investigation of two kinds of thin film dispersion freeze-dried vaccine liposome powder immunogenicity; The fourth chapter for the research progress of liposome as vaccine adjuvants (review).Experimental results show that the cationic liposome membrane dispersion method, the average particle size (including2.78m, the neutral liposomes of thin film dispersion method, the average particle size (including3.02m, with the average grain size of cationic liposome is neutral tend to be more uniformity, the average particle size of liposomes membrane dispersion lyophilization of cationic liposome vaccine flat and more than94.9%(including m particle size of1.0～7.0, the encapsulation rate was81.67%. Immune serum IgG levels, abdominal immune2%DC-Chol (3beta [N-(N, N-dimethyl amine oxide)’-amino formyl] cholesterol hydrochloride) liposome cationic liposome group as a whole than liposome neutral liposomes group. Pulmonary immune cationic liposome group1%DC-Chol, liposome conditions at low doses higher than neutral liposomes group of liposome; Pulmonary immune cationic liposome group (1%,2%,4%) DC-Chol, liposome conditions produce antibody level high doses group was better than neutral liposomes liposome. Cationic liposome is superior to the neutral liposomes, explain the immunogenicity of cationic liposome is superior to the neutral liposomes.