| [Objective] A study was carried out in the effect of Rb1(Panax notoginsenoside-Rb1, PNS-Rb1) on the abnormal microenvironment by observing the changes of regional cerebral blood flow, rCBF on rat hippocampal CA1area, and the expression of glial glutamate transporter1(glial glutamate transporter1, GLT-1), vascular endothelial growth factor (vascular endothelial growth factor, VEGF) and nestin (Nestin). Through investigating the influence and mechanism of PNS-Rb1in improving hippocampus abnormity microenvironment in rat, the study is assumed to provide the experimental evidence for further studying PNS-Rbi in the treatment of ischemic cerebrovascular disease.[Method]96male SD rats were randomly divided into16groups, n=6:Perfusion sham group, perfusion model group, after the perfusion to the high-dose PNS-Rb1group (100mg/kg), after the perfusion to the middle-dose PNS-Rb1group (50mg/kg), after the perfusion to the low-dose PNS-Rb1group (25mg/kg), perfusion after to high-dose nimodipine group (1mg/kg), perfusion after to middle-dose nimodipine group (0.5mg/kg), perfusion after to low-dose nimodipine group (0.25mg/kg), cerebral ischemia sham group, cerebral ischemia model group, after the cerebral ischemia to the high-dose PNS-Rb1group (100mg/kg), after the cerebral ischemia to the middle-dose PNS-Rb1group (50mg/kg), after the cerebral ischemia to the low-dose PNS-Rb1group (25mg/kg), cerebral ischemia after to high-dose nimodipine group (1mg/kg), cerebral ischemia after to middle-dose nimodipine group (0.5mg/kg), cerebral ischemia after to low-dose nimodipine group (0.25mg/kg). Copied the hippocampus abnormal microenvironment of rats model:a. Perfusion Model; b. Cerebral ischemia model. Each group was used intraperitoneal administration in4h after modeling. The rCBF in rat hippocampus observation:this article adopted Laser Doppler Flowmetry to observe the change of hippocampus rCBF after operation24h in the stress state. Immunohistochemical observation:after perfusion fixation, removed brain tissue, paraffin-embedded brain tissue, in the hippocampus of the serial sections (4μm), adopted immunohistochemisty to assay the expression of GLT-1, VEGF and Nestin under stress conditions.[Result]1. rCBF:Observed abnormal brain microenvironment after4h, finded high, medium and low doses of PNS-Rb1may increase hippocampal rCBF (P<0.05), in which the high-dose PNS-Rb1group increased significantly.2. GLT-1, VEGF, and Nestin immunohistochemical results:Perfusion model and cerebral ischemia model, PNS-Rb1high-dose group, it’s the strongest positive expression of GLT-1, VEGF, and Nestin in hippocampal CA1area. Average gray value was lowest, and the pyramidal cells were loosely arranged, PNS-Rb1high-dose group compared with the model group, the difference was statistically significant (P<0.05). In the medium dose group of PNS-Rb1, the number of positive cells of hippocampal CA1area on GLT-1, VEGF and Nestin reduced, compared with the model group, the difference was statistically significant (P<0.05). The medium, low-dose group of PNS-Rb1compared with the high-dose group significantly different (P<0.05), high dose group of PNS-Rb1hippocampal CA1region had the largest number of positive cells. The overall trend shows PNS-Rb1can regulate the expression of GLT-1, VEGF, and Nestin.[Conclusion] PNS-Rb1can increase rCBF and up-regulate the expression of GLT-1, VEGF, and Nestin as well. The possible mechanism can be that, firstly, the use of PNS-Rb1can increase cerebral blood flow after brain damage; secondly, with the rising of cerebral blood flow, nutrients will increase, which up-regulate the expression of GLT-1, VEGF, and Nestin, and to some extent improve the hippocampal abnormalities microenvironment of rat; thirdly, when a protective role on neuron injury and in the treatment of cerebral ischemia eventually is achieved, the experimental evidence for a further development of PNS-Rb1in the treatment of ischemic cerebrovascular disease can be provided. |
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