MRI Quantitative Assessment Of Intrahepatic Fat Content And Islet β Cell Function In Children With Non-alcoholic Fatty Liver Disease

Part1Study of islet βcell function in obese children with nonalcoholic fatty liver disease.Objectives:To investigate islet βcell function in obese children with nonalcoholic fatty liver disease.Methods:Three hundred and sixty-two obese children and one hundred and sixty-four normal children were enrolled in the study form June2007to April2011.170of the obese children accompanied with NAFLD,192simple obese children without NAFLD. Triglycerides、cholesterol、fasting plasma glucose(FPG)、fasting insulin(FINS)、HbAlc were assayed in these children. Oral glucose tolerance test and insulin releasing test were performed in all obese children. The homeostasis model assessment-insulin resistance(HOMA-IR),insulin sensitivity index (QUICKI) and P-cell function index (HOMA-β) were calculated.Results:FPG、FINS、HbAlc、HOMA-p and HOMA-IR in NAFL group were significantly higher than the other two groups(P<0.05); QUICKI in NAFLD group was significantly lower than the other two groups (P<0.05). The relevance ratio of abnormal glucose metabolism in NAFLD group was significantly higer than without NAFLD group(P<0.05).conclusion:Children with nonalcoholic fatty liver disease existed impaired function of islet β-cell and glucolipid metabolism disorders. The impaired function of islet β-cell could be one of the pathogenesis for nonalcoholic fatty liver disease.Part2To quantitatively evaluate clinical significance of intrahepatic fat (IHF) content in children with non-alcoholic fatty liver disease (NAFLD).Methods:Fifty-six obese children were enrolled in this study. Physical parameters, liver function, serum lipids, glycemic and insulin related parameters were measured. Liver B-mode ultrasound (US) examination was performed. IHF content was quantified by gradient echo duo-echo technique. Two subgroups were classified according to the conditional diagnostic criteria for obese children: obesity without NAFLD (n=24), NAFLD (n=32). IHF content among the two groups was compared. The relationship of IHF content with other common clinical laboratory parameters and independent factors influencing increased IHF content were investigated.Results:There were significant differences in IHF content between the groups. Univariate correlation analysis demonstrated that IHF content was positivelycorrelated with waist circumference, hip circumference, waist-to-hip ratio, body mass index, systolic blood pressure, diastolic blood pressure, alanine aminotransferase, aspartate aminoreansferase, y-glutamic acid transtetase, triglyceride, low-density lipoprotein, OGTT2-hour plasma glucose, fasting insulin,2-hour insulin and insulin resisfence, and negatively correlated with high-density lipoprotein.Conclusions:IHF content determined of obesity without NAFLD is hinger than NAFLD. There is a significant correlation between most of common clinical laboratory parameters and IHF content.