Quantitation Of Ferulic Acid In Danggui-Shaoyao-San And Pharmacokinetic Study Of Ferulic Acid After Oral Adminstration Of Danggui-Shaoyao-San And Pure Ferulic Acid

ObjectiveDanggui-Shaoyao-San from "Synopsis of Golden Chamber",a book of Zhang Zhongjing, containing Angelica sinensis (Oliv.) Diels root, Paeonia lactiflora Pall, root, Poria cocos (Schw.) Wolf fungus nucleus, Astractylodes macrocephala Koidz. root and rhizome, Ligusticum chuanxiong Hort. rhizome, Alisma orientale (Sam.) Juzep. rhizome, previously mainly used in the treatment of gynecological pain syndrome, have been discovered in recent years on the role of the treatment of AD and VD, beause of its pharmacological effects of anti-nerve inflammation, regulate neurotransmitters, anti-oxidation, etc. Ferulic acid is one of the main components of Angelica sinensis (Oliv.) Diels root, and Ligusticum chuanxiong Hort. rhizome, and its pharmacological effects is as the same as Danggui-Shaoyao-San on anti-nerve inflammation, anti-oxidation effects, in addition to regulate blood lipids, blood pressure and other effects, in resulting of treating neurodegenerative diseases cardiovascular disease, diabetic complications and other diseases, and being considered as the main prescription of the medicinal ingredient.This study will focus on how to determine ferulic acid in Danggui-Shaoyao-San and the pharmacokinetics of ferulic acid in rats after oral adminstration of pure ferulic acid and Danggui-Shaoyao-San.MethodsEstablish a Hplc-UV detection method for the determination of ferulic acid in Danggui-Shaoyao-San and verify the specificity, accuracy, precision, stability and et al., following determination of ferulic acid in the extract of Danggui-Shaoyao-San. After oral adminstration of Danggui-Shaoyao-San (ferulic acid,0.3mg/kg) and ferulic acid monomers (50mg/kg), the blood was collected from retinal venous plexus at the points of0.083、0.167、0.333、0.5、0.75、1、1.5、2、4、6h. After centrifugation,200ul plasma was collected in a1.5ml tuber which contented internal standard, and50ul hydrochloric acid (0. lmol/ml) and1ml ethyl acetate were added. The mixture was vortexed for2min, and centrifuged at12, OOOrpm for15min to obtain the supernatant which was then transferred into a clean EP tube, evaporated to dryness under a stream of nitrogen at40, and dissolved in methanol of50ul. Reconstituted material of20ul was injected to determine the concentration of ferulic acid in plasma, on the basis of which the pharmacokinetic parameters were obtained with the software of3P87.ResultsA rapid, accurate, reliable and stable method was established to determine the content of ferulic acid in Danggui-Shaoyao-San, and the content of ferulic acid in Danggui-Shaoyao-San was0.088%o. After oral adminstration of Danggui-Shaoyao-San, the pharmacokinetic parameters were of ferulic acid of Cmax, Tpeak, AUC, MRT, K (Terminal) were20.24±2.22ng/ml,0.28±0.096h,16.26±1.14(ng/ml)*h,0.49±0.01h,0.46±0.101/h, respectively. After oral adminstration of pure ferulic acid, the pharmacokinetic parameters of Cmax, Tpeak, AUC, MRT, K(Terminal) were43.65±13.35ng/ml,0.17±0h,19.19±5.03(ng/ml)*h,0.68±0.13h and0.14±0.0551/h, respectively.ConclusionThe experimental established a sample and rapid method for the determination of ferulic acid in a Dangguishaoyaosan, and successfully applied to the determination of ferulic acid. This experiment also studied the pharmacokinetics of fDanggui-Shaoyao-San and ferulic acid monomer in rats to provide experimental data for further pharmacokinetics study.