Comparative Study On Chemical Constituents And Pharmacology Between Radix And Stem Of Gangmei

ObjectiveTo compare the similarities and differences of chemical constituents and pharmacodynamics between the root and stem of Ilex asprella(Gangmei), and study the spectrum-effect relationship, so as to provide theoretical foundation and scientific basis for the rational use of root and stem.Method:1Fingerprint spectrum study of Gangmei1.1. TLC fingerprint spectrum study of GangmeiBuilt TLC fingerprint spectrum of Gangmei, and compared the similarities and differences of chemical constituents between the root and stem of Gangmei.1.2. HPLC fingerprint spectrum study of GangmeiBuilt HPLC fingerprint spectrum of Gangmei, and compared the similarities and differences of chemical constituents between the root and stem of Gangmei.2Pharmacodynamics comparative study of root and stem of Gangmei2.1Comparative study on the vitro antioxidant effectUsed three method of vitro test to compare the antioxidant capability of alcohol extraction of Gangmei.2.2Comparative study of Treating PneumoniaLet the mice inhale ammonia, to establish a mice model of pneumonia, and given the extract of root and stem of GangMei, compared the similarities and differences on pharmacodynamics effect.2.3Comparative study of Treating PharyngitisInjected solution of LPS, to establish a mice model of pharyngitis, and given the extract of root and stem of GangMei, compared the similarities and differences on pharmacodynamics effect.3. Comprehensive evaluationCombined with the result of fingerprint spectrum study and pharmacological experiments, used correlation analysis tools (such as PCA, HCA, GRA), to study the spectrum-effect relationship, compare the similarities and differences between the root and stem of Gangmei.Result1Fingerprint spectrum study of Gangmei1.1. TLC fingerprint spectrum study of GangmeiTLC result showed that the roots’chemical composition is similar to the stems’;The chemical composition of roots and the stems of Gangmei from different habitats are similar.1.2. HPLC fingerprint spectrum study of GangmeiThe samples of root and stem were collected from the same plant. Compared of chromatographies, there were some similarities and differences between root and stem. In the5-145min retention time range, There were16characteristic peaks belonged root while18belonged stem;They had12common peak.2. Pharmacodynamics comparative study of root and stem of Gangmei2.1Comparative study on the vitro antioxidant effectBoth of them had significant reducibility and hydroxyl radical scavenging ability,but with some differences in drug efficacy.2.2Comparative study of Treating PneumoniaThe mice which inhaled ammonia can be pneumonia, after given the extract of root and stem of GangMei, it can be significantly reduce the lung index. There was no significant difference in the efficacy between root and stem.2.3Comparative study of Treating PharyngitisThe mice which injected with LPS solution can be pharyngitis, after given the extract of root and stem of GangMei, it can be significantly relieved the pathological symptoms of pharyngitis, and had an obvious inhibitory effect on increasing NO, MDA content in serum of pharyngitis. There was no significant difference in the efficacy between root and stem.3. Comprehensive evaluationPharmacological effects of Gangmei was related to the various components in the root and stem of Gangmei. Their pharmacodynamic substance were not all the same.In the root, pharmacodynamic substances of antioxidant were mainly for P5、 P9、GB、P6、P10, while stems mainly as JA、P5、P6、P9、P2、P4、P10、P5、P9in Stems were more than in roots, which had significant antioxidant ability.In the root, pharmacodynamic substances for the treatment of pharyngitis were mainly for GA、P1、P4、P12, while stems mainly as P2、P4、P9、P10、P12、JD、 JE、JF. it suggested that substance and the mechanism for anti pharyngitis effective were not the same.GonclusionThere are differences between Gangmei root and stem in the chemical composition. Difference between the two anti-inflammatory mechanisms but achieve the same efficacy, so the combination has a certain rationality.