Study On The Anti-inflammatory And Antimicrobial Spectrum-effect Relationship Of Cyclocarya Paliurus(batal.) Iljinsk

Cyclocarya paliurus(Batal.)Iljinsk belongs to the Juglandaceae family which grows in cloudy and foggy forests between 500 and 2500 meters above sea level and only present in southern China.C.paliurus has obvious hypoglycemic,hypotensive,antioxidant and enhancing immune function and other effects,so it is known as the third tree in the medical field.At present,there is no research on the anti-inflammatory and antimicrobial spectrum-effect relationship of C.paliurus,so it is carried out in this paper.Ⅰ The identification of chemical constituents of C.paliurus.Based on related literature and chemical constituents of C.paliurus,the chemical constituents were identified by HPLC and LC-MS,compared retention time,UV absorption spectrum,relative molecular mass and ion debris of reference and sample.Seven compounds were identified from 10 batches of C.paliurus,including protocatechuic acid,chlorogenic acid,vanillic acid,isoquercitrin,quercitrin,quercetin and kaempferol.For the above seven compounds,an HPLC method was established for the simultaneous determination of seven compounds in C.paliurus.The results showed that the method was simple,accurate and reliable,and could be used for the determination of 7 chemical components in C.paliurus from different habitats.Ⅱ The fingerprint of C.paliurus.In this study,the fingerprint conditions of 10 batches of C.paliurus from different areas were established,19 common characteristic peaks were selected,and 7 of them were identified as protocatechuic acid,chlorogenic acid,vanillic acid,isoquercitrin,quercitrin,quercetin,kaempferol.The results of similarity analysis show that 8 of 10 batches of C.paliurus from different habitats have higher similarity.SPSS software was used for clustering analysis,and the results were consistent with the similarity analysis.According to the clustering analysis results,10 batches of C.paliurus from different origins are divided into two categories: A and B.Class A could be divided into A1 and A2 two subclasses.A1 contains C.paliurus from Sichuan,Wuhan,Yichang and Yunnan;A2 contains C.paliurus from Jiangxi,Zhangjiajie,Dabie Mountains and Guangxi;B contains C.paliurus from Guizhou and Hebei.Ⅲ The study on the relationship between anti-inflammatory and antibacterial spectrum of C.paliurus.In vitro bacteriostatic and anti-inflammatory experiments were used to compare the anti-inflammatory and antibacterial activities of C.paliurus from different habitats by using the inhibition zone size,nitric oxide(NO),antitumor necrosis factor-α(TNF-α)and interleukin-6(IL-6).The results show that C.paliurus from different habitats have different anti-inflammatory and antibacterial activities.Therefore,combined with the fingerprint,principal component analysis,grey correlation analysis and partial least squares regression analysis were used to explore the spectral effect relationship.The results of principal component analysis demonstrate that the cumulative contribution rate to variance is 93.163% when the first three principal components of 19 common peaks are extracted,which is more than 85%.Peaks5,6,7,8,9 and 11 make the largest contribution to principal components,followed by peaks 1,2,4 and 12.The results of grey correlation analysis indicate that each peak has a large grey correlation,and the 1st,9th,10 th,12th and 14 th peaks have a large grey correlation.The results of partial least squares regression analysis show that the absolute value of standardized coefficient of anti-inflammatory activity of peaks 5,10 and 15 are the largest,which are the main pharmacodynamic peak;and the absolute value of standardized coefficient of antibacterial activity of peaks 2,8,15 and 19 are the largest,which are the main pharmacodynamic peak.According to the results of principal component analysis,grey correlation analysis and partial least squares regression analysis,the results demonstrate that peaks 5,8 and 12 make the most contribution to the anti-inflammatory activity,and peaks 1,4,6,9,11 and15 make the most contribution to the antibacterial activity,among which peak1 is protocatechuic acid,peak 4 is chlorogenic acid,peak 5 is vanillic acid,peak9 is isoquercitrin,and peak 11 is vanillic acid Quercitrin.The results indicate that the effective substances of C.paliurus are probably the above compounds,which provide scientific support for the formulation of scientific,standardized and reasonable quality standards.In conclusion,the research on the identification of chemical components,fingerprint as well as anti-inflammatory and antimicrobial spectrum-effect relationship of C.paliurus show that the effective substances of this medicine may be protocatechuc acid,chlorogenic acid,vanillic acid,isoquercetin and quercetin,which provides scientific theoretical basis for the development and utilization of C.paliurus.