Effect Of Modified Youguiyin On Rabbit Cartilage Cell Apoptosis In Osteoarthritis Of Knees And The Regulation Of Gene Expression Of Bax、Bcl-2

Objective：Establishing the model of rabbit bone of knee arthritic,observation ofmodified Youguiyin effect on the model of cartilage cell apoptosis and Bcl-2,Baxgene expression,and to explore the mechanism of modified Yougui Yin in treatingknee osteoarthritis.Method: Choose48Japanese big ear rabbits and randomly divided into4groups,group A as normal control group, groupa B as model control group, group C astreatment control group(celecoxib), group D as Modified Youguiyin group,with12rats in each group. B, C, D group with medical polymer materials fixed rabbit leftknee in extension for4weeks, to establish animal model of osteoarthritis, determinethe first day after beginning to take medicine. The A group were fed with normal diet;B group were fed with Standard diet; group C was fed with the celecoxib (accordingto75mg/D administration, mixed in the Standard diet);group D was fed withModified Youguiyin (including Modified Youguiyin ultrafine powder120g/Dadministration, mixed in the Standard diet). In eight weeks after modeling with earvenous air embolism method kill all the experiment rabbit,remove the left posteriorknee joint cartilage(in order to reduce the man-made difference,all specimens aretaken from the femoral condyle articular cartilage),test with gross observation、lightand electron microscopic analysis、cartilage cell apoptosis and the gene expressionof Bax、Bcl-2.Result: The experimental results show that group C、group D in both inhibitchondrocyte apoptosis trend played a certain extent, in inhibiting the expression of apoptosis promoting gene Bax and apoptosis suppressor gene Bcl-2are better thangroup D, there are significant differences between the group C and group D.Conclusion:(1)Modified Yougui must play an positive effect in maintainingmorphological and functional of the rabbit cartilage integrity, which can increaseeffectively inhibits osteoarthritis;(2)Modified Yougui could significantly decrease theapoptosis of osteoarthritis of the rabbit knee joint cartilage cell rate, and to protect thearticular artilage cells, Delay the occurrence and development of disease.(3) ModifiedYougui Yin intervention some genetic pathways in capoptosis of osteoarthritis ofrabbit knee joint cartilage cell:①also means it can promote the expression ofinhibitory effect of gene on the trans membrane bax,to inhibit the expression of Bax,apoptosis and inhibition of cartilage cells;②it also can promote the expression of antiapoptosis gene bcl-2,may synergistically increased the expression of bcl-2, apoptosisof cartilage cell from another way.Study found out that celecoxib intervene in theapoptosis of osteoarthritis of knee joint cartilage cell gene pathways that have obviousdifference.