| Objective:To study the expression of CXCL8and its receptors CXCRl and CXCR2in PMNs of peripheral blood of Chronic Hepatitis B. Observe the influence to the expression of CXCL8,CXCR1and CXCR2in PMNs after HBV infection.Methods:Typical patients of42cases of chronic Hepatitis B, in which,17cases were HBsAg(+),25cases were HBsAg(-),15cases were HBV-DNA(+) and29cases were HBV-DNA(-). Levels of CXCL8in serum were detected by ELISA. mRNA of CXCL8, CXCR1and CXCR2in PMNs were detected by qPCR after total RNA was extracted and reverse transcripted into cDNA. The levels of mRNA were represented by lgcDNA/lgGAPDH. The correlation of levels of CXCL8in serum and expression of mRNA of CXCL8, CXCR1and CXCR2in PMNs with ALT in serum were also observed. Expression of proteins of CXCL8, CXCR1and CXCR2in PMNs were detectved by SABC immunocytochemistry, evaluate the expression of CXCL8, CXCRl and CXCR2in PMNs after infection of HBV.Results:The level of CXCL8in serum of CHB was (432.43±216.33) pg/mL.In which, there was significant difference between the levels of CXCL8in HBeAg(+) and HBeAg(-) were (612.15±165.56) pg/mL and (310.21±152.42) pg/mL(P<0.01), as well as the levels of CXCL8in serum of CHB with HBV-DNA(+) and HBV-DNA(-) CP<0.01). The levels of mRNA of CXCL8and CXCR1in PMNs of CHB were1.12±0.21and0.86±0.37, which were significant higher than those in controls (P<0.01). In which, the levels of mRNA of CXCL8and CXCR1in HBeAg(+) were (1.54±0.30) and (1.01±0.34), which were significant higher than those in HBeAg(-)(P<0.05). The levels of CXCR2mRNA in HBeAg(+) and HBeAg(-) were1.21±0.15and1.13±0.32, there was no significant difference between them (P>0.05). The levels of mRNA of CXCL8and CXCR1in HBV-DNA(+) and HBV-DNA(-) were (1.22±0.26,1.05±0.21) and (1.08±0.18,0.79±0.42) respectively (P<0.05), but not the CXCR2mRNA(P>0.05). There was significant correlation between CXCL8and ALT in serum (r=0.859, P<0.01), as well as the levels of mRNA of CXCL8and CXCR1in PMNs with ALT in serum (r=0.688, P<0.01;r-=0.585, P<0.01),but not the CXCR2mRNA (r=0.088, P>0.05).SABC immunocytochemistry showed that the CXCL8mainly in the cytoplasm of PMNs,while the CXCR1and CXCR2seen in the cytoplasm and cell membrane. In which, the CXCL8and CXCR1in HBeAg(+) were deep in color, while the CXCR2was coloring shallow, as well as which in HBV-DNA(+) and HBV-DNA(-). The expression of CXCL8and CXCR1in CHB were significant difference with controls (P<0.05), but not the CXCR2(P>0.05).Conclusions:HBV is ecotropic viru for liver cells, it can also infect PMNs and cause extrahepatic infection, which maybe the main etiology of recurrent and refractory of CHB. The expression of CXCL8in serum and the mRNA of CXCL8, CXCR1and CXCR2in PMNs were increased. There were positively correlated relationship between the levels of ALT in serum and the expression of CXCL8in serum, as well as the mRNA of CXCL8and CXCR1in PMNs. HBV can interfere the host cell metabolism, increasing the secretion of CXCL8and expression of CXCR1and CXCR2in cytomembrane after infecting PMNs. CXCL8mainly located in cytoplasm of PMNs, while the CXCR1and CXCR2were seen in cytoplasm and cell membrane, the dyeing degree of the three were closely related to the expression of HBeAg and HBV-DNA. The secretion of CXCL8in cytoplasm and expression of CXCR1and CXCR2in cell membrane were further increased after HBV infecting PMNs. The PMNs which overexpressed CXCR1and CXCR2interact with CXCL8, attract more PMNs to lesions and Participate in the local inflammatory and tissue repair. |
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