In Vivo Tracking Of Three Transplanted Routes Of MSCs In A Mice Of Acute Hepatic Injury Model By IVIS

Objective:We used in vivo imaging system(TVIS) to trace the migration, distribution, colonization of MSCs in mice model of acute hepatic injury.Methods:1. Thawed and subcultured the labeled of GFP-Luc MSCs;2. Fourty-eight male C57BL/6mice were selected;3.Carbon tetrachloride was used to bulid mice model of acute hepatic injury.4.Mice were grouped as follows:tail vein group(8mice), portal vein group (8mice), intrahepatic group(8mice) and control group(8mice);5.MSCs were transplanted into acute hepatic injury mice through three routes:tail vein, portal vein and intrahepatic.6. the whole body and organs of mice in vivo and vitro were imaged by IVIS at the6st hour,24st hour,72st hour,120st hour and168st hour after transplantation to see MSCs colonization in mice.Results:1. The character of MSCs were stable after thawing and subculturing; The fluorescence intensity increased with the concentration of GFP-Luc-MSCs;2.we successfully set up the acute hepatic injury mice model, and improved the equilibrium and stability of the model;3. We successfully transplanted MSCs by three routes;4. IVIS tracing showed that no fluorescent expression in the liver was detected by tail vein transplantation, but we can see fluorescent expression in the tail and lung of the mice. Moreover, IVIS tracing showed that fluorescent expression was detected in the liver by portal vein and intrahepatic transplantion;5.The number of fluorescent photo flux in portal vein group is higher than intrahepatic group. However, it has no significantly difference between portal vein group and intrahepatic group(P>0.05).Conclusions:MSCs transplantation through portal vein and intrahepatic routes can colonize in the injured hepatic of mice.