| Objective:To establish New Zealand rabbit by high-fat diet nonalcoholic fatty liver disease (NAFLD) model, to investigate the therapeutic effect of Danning tablet on the NAFLD of New Zealand white rabbits and the possible mechanism.Methods:Selection30New Zealand rabbits, body weight2-2.50kg, half male and half female, ordinary forage adaptability feeding after1weeks of feeding, were randomly divided into3groups. The control group (8rats):the daily feeding100g ordinary particles feed, and then27.5ml physiological saline, model group (10rats):Daily fed with high cholesterol diet containing1.2g100g, and then27.5ml physiological saline, Danning tablet group (12rats): daily feed containing1.2cholesterol diet g100g, and to27.5ml Danning tablet by gavage. The above three groups of feeding30days after fasting for12h, no water for6h, the carotid artery blood, open, quickly take liver tissue is preserved in liquid nitrogen. Detection of total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-C), low density lipoprotein (LDL-C), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transpeptidase (γ-GT), alkaline phosphatase (ALP) and LDH (lactate dehydrogenase). To observe the pathological changes of hepatic tissues stained with HE, RT-PCR in liver tissue of hepatic cytochrome P4502E1enzyme (CYP2E1) expression and cholesterol7alpha hydroxylase (CYP7A1) expression.Results:HE staining showed that nonalcoholic fatty liver model was constructed successfully; TC numerical control group, model group and Danning tablet group were15.60±3.60,35.9±9.29mg/dl,21.37±6.03mg/dl, TG were0.86±0.41,1.57±0.46mmol/L,0.96±0.29mmol/L, LDL-C were16.84±8.08,17.36±4.04mmol/L,11.18±4.41mmol/L, HDL-C were1.22±0.51mmol/L,0.76±0.29mmol/L,0.92±0.55mmol/L, comparison of Danning tablet group and the model group, serum index, there was statistically significant difference (P<0.01), model group of New Zealand rabbit liver tissue CYP7AlmRNA expression were Danning tablet group (model group and Danning tablet group. With values were0.56±0.14,1.02±0.09), the difference was statistically significant (P<0.001). The expression of CYP2E1mRNA in model group was more Danning tablet group (model group and Danning tablet group with values were1.49±0.31,1.22±0.20), the difference was statistically significant (P＜0.05). HE staining showed that the liver pathological changes improved compared with model group; RT-PCR results show the model group of New Zealand rabbit liver tissue CYP7A1mRNA expression were Danning tablet group, the difference was statistically significant (P<0.001), and the expression of CYP2ElmRNA in model group was more Danning tablet group, the difference was statistically significant (P<0.05). Conclusion:1.consecutive high fat diet for4weeks of feeding the successful construction of New Zealand rabbit model of non alcoholic fatty liver.2.Danning tablet can improve lipid metabolism, protect the liver cell function and structure, prevent cholesterol accumulation, reduce lipid peroxidation, improved hepatic inflammation and steatosis, the New Zealand rabbit NAFLD has preventive and treatment effects of good3.Danning tablet could improve the disorder of lipid metabolism, anti-inflammatory, and its mechanism may be related to Danning tablet can improve the expression of hepatic CYP7AlmRNA cells in the liver, promote the synthesis of cholesterol to bile acid, cholesterol and prevent excessive accumulation, decreased the expression of CYP2E1mRNA in liver, thus reducing the fatty degeneration and enhance the antioxidant ability of liver cells.4.Danning tablet has the potential to become drugs is a safe and effective treatment for NAFLD. |
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