Impact Of Echincoccus Granulosus Antigen B On NOD Mouse Pancreas Cytokine Expression

Objective:(antigen B) effects on non-obese diabetic (NOD) mice with type1diabetes, the immune mechanism. Methods:20female mice by intraperitoneal injection of body weight given65mg/kg STZ, construction NOD mouse model, using a random number table successful model of20NOD mice were divided into A, B groups, A group was given Echinococcus hydatid antigen B intraperitoneal injection, group B received the same dose of normal saline (NS), each10. Recorded and compared two groups of mice initial/molded after one week, two weeks, body weight and blood glucose values three weeks afters medication. The use of the expression level of fluorescence quantitative polymerase chain reaction (PCR) detection of pancreatic tissue of interleukin (IL-4) and interferon (IFN-γ) cytokines. Results:initial body weight in mice, body weight and body weight groups B antigens without treatment within two weeks after the successful model change significantly, no significant difference (P>0.05), the third week after treatment, mice were monitored weight display:B group of small rat body weight was significantly lower than that in group A, with statistical significance (P<0.05). A group of mice initial blood glucose levels were in the normal range, after successful modeling average blood glucose greater than16.7mmol/L, blood glucose levels after three weeks of treatment with a successful modeling compared to decline, at different time points during treatment (one week, two weeks, three weeks) no significant change in blood glucose levels after group B mice modeling success continued at a high level of blood glucose status, after three weeks of saline treatment, no significant decrease in blood glucose, the use of repeated measures analysis of variance showed that between groups different time points P<0.05, during the treatment, the mice in group B than in group A was significantly elevated blood sugar. Quantitative PCR results:antigen B treatment group IFN-γ mRNA expression level was significantly reduced compared with the control group, IL-4was significantly increased, with statistical significance(P<0.05). Conclusion:The experiment initially considered Echinococcus granulosus antigen B can reduce the level of IFN-γ, elevated levels of IL-4, tentatively considered so Th1/Th2immune deviation toward the direction conducive to the protection of islets, fine hydatid antigen B may have a preventive or therapeutic effect of type1diabetes.