Association Between Chromosome9p21Polymorphism And The Large-artery Atherosclerosis Stroke

Object:To explore the association between rs10757274which was the positive locus of GWAS and rs7049105, rs647188, rs1333035which were in the Block1of chromosome9p21and the LAS among Chinese Han population of Changsha; to explore the association between rs7049105, rs647188, rs1333035and the LAS among patients with preexisting atherosclerosis.Methods:The present study comprised229LAS patients with LAS and233"healthy" controls matched for age and gender.The233controls which we defined control group1. In the controls,150(64.38%) controls with preexisting atherosclerosis were defined as control group2,83(35.62%) controls withnot preexisting atherosclerosis were defined as control group3. The sample genotyping was detected using MALDI-TOF-MS. First, we compared the distribution of polymorphism between case group and control group1, and we used Meta-analysis to compare the association between rs10757274and LAS. Second, we compared the distribution of polymorphism between case group and control group1, control group2and control group3of rs7049105、 rs647188、rs1333035. Last, Logistic regression was used to find the influence factors of LAS.Results:1. The case group was compared with control group.there were no significant differences in sex and age (p>0.05),but differences in BMI, hypertension, diabetes history (p<0.05)2. The SNPs of rs7049105(G/A),rs1333035(T/C),rs10757274(A/G) existed polymorphism among case group and control group1. There is no polymorphism of rs647188because it had only allele A.3. The polymorphism distribution was no statistically significant difference (p>0.05) between the case group and control group of rs10757274. But when a Meta-analysis which including8studies (cases:5311, controls:18061) were conducted, rs10757274was associated with the risk of LAS in dominant model (OR=1.23,95%CI=1.08-1.41), recessive model (OR=1.28,95%CI=1.01-.1.61) and allele model (OR=1.10,95%CI=1.06-1.14).4. The polymorphism distribution between the case group and control group of rs7049105and rs1333035:①The polymorphism distribution was no statistically significant difference (p>0.05) between the case group and control group1and3of rs7049105and rs1333035.②The polymorphism distribution was no statistically significant difference (p>0.05) between the case group and control group2of rs7049105. The rs1333035was associated with risk of LAS among patients with preexisting atherosclerosis(p=0.039).The CC genotype may be a protective factor for LAS because rs1333035showed a correlation tendency (p=0.144, OR=0.382,95%CI=0.122-1.192) in the dominant model(CT+TT/CC).5. The Logistic regression model result showed that the multiplicative interaction effects of LAS include hypertension, diabetes, BMI. The rsl333035was not included.Conclusions:1. The rs1333035polymorphism in the chromosome9p21was associated with risk of LAS among Han population of Changsha with preexisting atherosclerosis. The CC genotype of rs1333035may be a protective factor for LAS among Han population of Changsha with preexisting atherosclerosis.2. The rs10757274and rs7049105polymorphism in the chromosome9p21were not associated with risk of LAS among Han population of Changsha. There is no polymorphism of rs647188among Han population of Changsha.