Research Of Rosiglitazone On Proliferation Of Human Cervical Carcinoma Hela Cells And Mechanisms

Objective:to investigate the proliferation effect of rosiglitazone(ROZ)on human cervical cancer cell line HeLa and to study the possiblemechanisms.Methods:The proliferation of HeLa cells were measured by MTTassay;the flow cytometry were used to evaluate the apoptosis and cellcycle,respectively;the expression of PTEN、p-Akt、CyclinD1protein inHeLa cells were investigated by Western-blot.Results:MTT analysis showed that, the tumor cell proliferation can besignificantly inhibited after ROZ treatment， and showed a time-anddose-dependent manner.Cell cycle analysis demonstrated that,rosiglitazone increased the proportion of cells in G1phase, S phase cellsdecreased and the cell apoptotsis rate was （6.24±1.5）%. ROZ enhancedthe expression of PTEN and inhibited the expression of p-Akt、CyclinD1protein.Conclusions:ROZ inhibited the cell proliferation and promotedapoptosis in vitro， which may be relative to enhanced the expression ofPTEN and inhibited the expression of p-Akt、 CyclinD1protein. Rosiglitazone is expected to become the therapeutic agent for the treatmentof cervical cancer.