Study On The Chemical Components And Extraction Technology Of Carthamus Tinctorius L

The chemical components along with the extraction technology of Carthamustinctorius L, have been studied in this dissertation.1. Air-dried C.tinctorius L (1.6kg) was extracted with75%ethanol every seven daysfor four times, after removing the solvents under reduced pressure, the residue (0.57kg)was obtained. The residue was suspended in water and then extracted successively withpetroleum ether, chloroform, ethyl acetate and n-butanol to give corresponding fourfractions. Three compounds were isolated and purified from chloroform part and ethylacetate part by chromatography with silica gel column, thin layer chromatography (TLC)and recrystallization technique. Based on spectral methods ultraviolet (UV), infrared (IR),mass spectrometry(MS),1H nuclear magnetic resonance (1H NMR), some physicochemicalmethods and data reported in references, three compounds were identified as Coumarin3-(3’,4’-dimethoxyphenyl)-7-hydroxyl-8-(3-methylbuthyl)(1),3-Pentone-1-(2-pyridiny-l)(2),Benzen-eacetic acid, α-methoxy-1-undecyldodecyl ester(3). All of them were isolated fromC. tinctorius L for the first time.2. The extraction technology of C. tinctorius L, referring to the content of hydroxysafflower yellow A, was determined by high performance liquid chromatography. Singlefactor and orthogonal design were applied and the solvent concentration, the solventconsumption, the time of the extraction, and the extraction temperature were inspected.Then by measuring the Rel.area of different condition, the optimum extraction conditionwas as follow: adding10times of70%ethanol extracted only once for20minutes under50℃, with ultrasonic.