The Hepatic Protection And Mechanism Of Action Of Rhein Lysinate In The Model Of Diabetic Rats

Objective To investigate the hepatic protection and molecular mechanism of rheinlysinate (RHL) on the model of diabetic rats, and to provide theoretical and experimentalbasis for RHL e treatment of diabetic metabolic liver damage.Methods1Diabetic rats model:40male SD rats (200±10g) were selected and wereadaptive feed a week later, the10was selected at random as normal control group, theremaining30as experimental group and was established diabetic rats model byintraperitoneal injection of chain urea rhzomorph.2Diabetic model rats were assigned tothe following three groups: model,25mg·kg-1and50mg·kg-1rhein lysinate-treatedgroup. The stomach of the25mg·kg-1and50mg·kg-1rhein lysinate-treated group werefilled with25mg·kg-1and50mg·kg-1RHL. And the stomach of the of the diabetic modelrats and normal control group rats were filled with the same amount of saline.the changeof the experimental rats weight was recorded everday.8weeks later, glucose oxidasemethod was used to detect the blood glucose value; biochemical analyzer was used todetect serum total cholesterol and triglyceride levels; The content of malonaldehyde(MDA)and the activities of superoxide dismutase (SOD) and glutathione peroxidase(GSH-Px) were detected by microplate reader according to the instruction of respectivekits. The pathological profile of liver tissue was observed by hematoxylin and eosin (HE)stain; the content of fat in liver tissue was observed by nile red stain; the changes ofproteins ERK1/2and SREBP-1c in fat synthesis were detected by western bloting method.Results1Compared to control group, the difference of the body weight, blood glucose,total cholesterol and triglyceride are statistically significant (P<0.05); there were a plentyof fat vacuoles and fat accumulation in liver tissues; the difference of MDA, SOD, GSH-Px, and protein expression levels of ERK1/2-SREBP-1c were statistically significant(P<0.05) in western bloting analysis.2Compared to model group, after the treatment of25mg·kg-1and50mg·kg-1RHL, the rats weight difference was not statisticallysignificant (P>0.05), but the difference of the blood glucose, total cholesterol andtriglyceride were statistically significant (P<0.05); the difference of MDA, SOD, GSH-Px, and protein expression levels of ERK1/2-SREBP-1c were statistically significant(P<0.05) in western bloting analysis.Conclusion1Diabetic model rats blood glucose, total cholesterol and triglyceride, the level of model rats with liver steatosis could be reduced by RHL, and the liver of diabeticrat could be sprotect by RHL.2This mechanism could be related to The effect ofoxidative stress, inhibiting liver steatosis and fat accumulation, inhibition of ERK1/2andSREBP-1c signaling pathways were inhibit by RHL.3RHL was expected to become anew drug to prevent the diabetic liver damage.