Establishment And Application Of Determination Method Of PAHs And Metabolites In Urine

Objectives This study focuses on a typical water pollution area in the Huaihe RiverValley region as a research site. Based on a case-control study:1By the establishment ofa GC-MS-MS detection method with high-sensitivity and high-throughput,detected theenvironmental contaminants PAHs and metabolites in the human urine,evaluated theexposure levels of human in the area;2By analyzing the differences of exposure levels ofPAHs between esophageal cancer cases and controls,the biological monitoring ofnvironmental contaminants PAHs was carried out.Methods Based on a case-control study, by the establishment of a high-throughput, high-sensitivity GC-MS-MS detection method to detect30kinds of PAHs and methyl,hydroxymetabolites in human urine of esophageal cancer cases and controls of the study area,carried out the biological monitoring of environmental contaminants PAHs, evaluated theexposure levels of PAHs in the study area. Use SPSS18.0for statistical analysis.Results1This study established a hexane liquid-liquid extraction combined with GC-MS-MS detection method to detect15PAHs,8PAHs hydroxy metabolites,7PAHsmethyl metabolites in human urine. LOD of15kinds of PAHs is0.02~0.25μg/L, LOQ is0.07~0.84μg/L, the recovery rate is77%~98%, inter-day precision is2.9%, intra-dayprecision is3.2%; LOD of8kinds of PAHs hydroxy metabolite is0.10~0.52μg/L, LOQ is0.33~1.74μg/L, the recovery rate is80%~90%, inter-day precision is3.6%, intra-dayprecision is4.2%; LOD of7kinds of PAHs methyl metabolite is0.05~0.78μg/L, LOQ is0.20~2.61μg/L, the recovery rate is77%~98%, inter-day precision is2.9%, intra-dayprecision is3.2%.2The exposure levels of naphthalene, acenaphthene, anthracene,fluoranthene, pyrene, benzo[g,h,i]perylene,2-hydroxy-fluorene,9-phenanthrenol,6-hydroxy-chrysens,1-methyl-pyrene of esophageal cancer cases were significantly higherthan controls. Stratified by age, in≥60group, the exposure levels of naphthalene,9-phenanthrenol were significantly higher than controls; in＜60group, the exposure levels ofnaphthalene, acenaphthylene, acenaphthene, fluoranthene, pyrene, benzo[g,h,i]perylene,2-hydroxy-fluorene,1-methyl-pyrene were significantly higher than controls.Conclusions1In this study, hexane liquid-liquid extraction combined with GC-MS-MSdetection method was established to detect PAHs and hydroxy, methyl metabolites in urine. This method comprising: the advantages of high-sensitivity, high-throughput, broad-spectrum, can be used for biological monitoring of large group people.2The exposurelevels of naphthalene, acenaphthene, anthracene, fluoranthene, pyrene,benzo[g,h,i]perylene,2-hydroxy-fluorene,9-phenanthrenol,6-hydroxy-chrysens,1-methyl-pyrene of esophageal cancer cases were significantly higher than controls,Stratified by age, in≥60group, the exposure levels of naphthalene,9-phenanthrenolwere significantly higher than controls; in＜60group, the exposure levels of naphthalene,acenaphthylene, acenaphthene, fluoranthene, pyrene, benzo[g,h,i]perylene,2-hydroxy-fluorene,1-methyl-pyrene were significantly higher than controls, suggesting that theincidence of esophageal cancer may be related with the exposure of PAHs.